Fibronectin type III domain-containing 4 promotes the migration and differentiation of bovine skeletal muscle-derived satellite cells via focal adhesion kinase

Cell Adh Migr. 2020 Dec;14(1):153-164. doi: 10.1080/19336918.2020.1810508.


FNDC4 is an anti-inflammatory factor that alters the activation state of macrophages; it is used to treat colitis in mice. However, its role in muscle formation and mechanism of function remains unknown. We found that FNDC4 promotes the bovine MDSCs migration and differentiation. Furthermore, we reported that it interacts with integrin β1 (ITGβ1). FAK, mediated by ITGβ1, regulates cell migration. Our results found FNDC4 to influence the expression of p-FAK, p-paxillin, and vinculin. Then, overexpressed or added FNDC4 protein could not influence migration and differentiation any more when the activated form of FAK was reduced. Therefore, we concluded that FNDC4 promotes the differentiation and migration of bovine MDSCs via the FAK, mediated by the ITGβ1 receptor.

Keywords: FNDC4; differentiation; focal adhesion kinase; migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cell Differentiation* / drug effects
  • Cell Movement* / drug effects
  • Fibronectin Type III Domain*
  • Focal Adhesion Protein-Tyrosine Kinases / antagonists & inhibitors
  • Focal Adhesion Protein-Tyrosine Kinases / metabolism*
  • Integrin beta1 / metabolism
  • Protein Kinase Inhibitors / pharmacology
  • Proteins / chemistry*
  • Proteins / metabolism*
  • Satellite Cells, Skeletal Muscle / cytology*
  • Satellite Cells, Skeletal Muscle / drug effects
  • Satellite Cells, Skeletal Muscle / metabolism*


  • Integrin beta1
  • Protein Kinase Inhibitors
  • Proteins
  • Focal Adhesion Protein-Tyrosine Kinases

Grants and funding

This work was supported by the Natural Science Foundation of heilongjiang Province (CN) [C2016029].