The enzymatic and mass spectrometric identification of 2-oxophytanic acid, a product of the peroxisomal oxidation of l-2-hydroxyphytanic acid

Biomed Environ Mass Spectrom. 1988 Mar 15;15(6):345-51. doi: 10.1002/bms.1200150607.

Abstract

A previously unreported metabolite of the mammalian phytanic acid breakdown pathway, 2-oxophytanic acid, was isolated and analysed by mass spectrometry. The metabolic origin of the 2-oxoacid is the oxidation by a rat kidney peroxisomal H2O2-generating oxidase of L-2-hydroxyphytanic acid, a well-established intermediate in phytanic acid alpha-oxidation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alcohol Oxidoreductases / analysis
  • Animals
  • Chromatography, Gas
  • Eicosanoic Acids / analysis*
  • Gas Chromatography-Mass Spectrometry
  • Kidney / metabolism*
  • Kidney Cortex / enzymology
  • Kidney Cortex / metabolism
  • Male
  • Microbodies / metabolism*
  • Oxidation-Reduction
  • Phytanic Acid / analogs & derivatives
  • Phytanic Acid / analysis*
  • Rats
  • Rats, Inbred Strains

Substances

  • Eicosanoic Acids
  • Phytanic Acid
  • 2-hydroxyphytanic acid
  • 2-oxophytanic acid
  • Alcohol Oxidoreductases
  • 2-hydroxyphytanate oxidase