Arthrobacter mobilis sp. nov., a novel actinobacterium isolated from Cholistan desert soil

Int J Syst Evol Microbiol. 2020 Oct;70(10):5445-5452. doi: 10.1099/ijsem.0.004431. Epub 2020 Sep 4.


A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity). The peptidoglycan was of the A3α type and the whole-cell sugar profile was found to contain galactose. The major menaquinone was MK-9(H2). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids. The major fatty acids identified were anteiso-C15 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 68.69 mol%. The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively. On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp. nov. is proposed. The type strain of Arthrobacter mobilis is E918T (=JCM 33392T=CGMCC 1.16978T).

Keywords: Actinobacteria; Arthrobacter; desert soil; taxonomy.

MeSH terms

  • Arthrobacter / classification*
  • Arthrobacter / isolation & purification
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Desert Climate*
  • Fatty Acids / chemistry
  • Glycolipids / chemistry
  • Nucleic Acid Hybridization
  • Pakistan
  • Peptidoglycan / chemistry
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry


  • DNA, Bacterial
  • Fatty Acids
  • Glycolipids
  • Peptidoglycan
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 9