Your Base Editor Might Be Flirting with Single (Stranded) DNA: Faithful On-Target CRISPR Base Editing without Promiscuous Deamination

Scott P Collins; Chase L Beisel

doi:10.1016/j.molcel.2020.07.030

Your Base Editor Might Be Flirting with Single (Stranded) DNA: Faithful On-Target CRISPR Base Editing without Promiscuous Deamination

Mol Cell. 2020 Sep 3;79(5):703-704. doi: 10.1016/j.molcel.2020.07.030.

Authors

Scott P Collins¹, Chase L Beisel²

Affiliations

¹ Department of Chemical & Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
² Department of Chemical & Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA; Helmholtz Institute for RNA-based Infection Research (HIRI) / Helmholtz Centre for Infection Research (HZI), 97080 Würzburg, Germany; Medical Faculty, University of Würzburg, 97080 Würzburg, Germany. Electronic address: chase.beisel@helmholtz-hiri.de.

PMID: 32888434
DOI: 10.1016/j.molcel.2020.07.030

Abstract

Jin et al. (2020) engineered new variants of CRISPR base editors that make precise genomic edits in rice protoplasts while minimizing untargeted mutagenesis.

Publication types

Comment

MeSH terms

CRISPR-Cas Systems
Clustered Regularly Interspaced Short Palindromic Repeats*
Cytosine
DNA, Single-Stranded
Deamination
Gene Editing*

Substances

DNA, Single-Stranded
Cytosine

Grants and funding

R35 GM119561/GM/NIGMS NIH HHS/United States