Acacetin improves endothelial dysfunction and aortic fibrosis in insulin-resistant SHR rats by estrogen receptors

Mol Biol Rep. 2020 Sep;47(9):6899-6918. doi: 10.1007/s11033-020-05746-3. Epub 2020 Sep 6.

Abstract

The aim of the work was to investigate the effects of acacetin on endothelial dysfunction and aortic fibrosis in insulin-resistant SHR rats and explore its mechanism. Seven-week-old male spontaneously hypertensive rats (SHR) were selected to establish a rat model of hypertension with insulin resistance induced by 10% fructose. The nuclear factor kappa B p65 (NF-κB p65) and Collagen I were observed by Immunohistochemistry. Immunofluorescence was used to observe estrogen receptor-alpha (ERα), estrogen receptor-beta (ERβ), and G protein-coupled receptor 30 (GPR30). Western blotting was used to detect interleukin (IL-1β), Arginase 2 (ARG2), Nostrin, endothelial nitric oxide synthase (eNOS), TGF-β, Smad3, ERK pathway proteins such as p-c-Raf, p-MEK1/2, p-ERK, ERK, p-P90RSK and p-MSK1. We found that acacetin did have an improvement on endothelial dysfunction and fibrosis. Meanwhile, it was also found to have a significant effect on the level of estrogen in this model by accident. Then, the experiment of uterine weight gain in mice confirmed that acacetin had a certain estrogen-like effect in vivo and played its role through the estrogen receptors pathway. In vitro experience HUVEC cells were stimulated with 30 mM/L glucose and 100 mM/L NaCl for 24 h to establish the endothelial cell injury model. HUVEC cells were treated with 1 μM/L estrogen receptors antagonist (ICI 182780) for 30 min before administration. Cell experiments showed that acacetin could reduce the apoptosis of HUVEC cells, the levels of inflammatory cytokines and the expression of TGF-β, Collagen I and Smad3 in endothelial cell injury model. After treatment with ICI 182780, the improvement of acacetin was significantly reversed. The results showed that acacetin relieved endothelial dysfunction and reduced the aortic fibrosis in insulin-resistant SHR rats by reducing the release of inflammatory factors and improving vasodilatory function through estrogen signaling pathway.

Keywords: Acacetin; Aortic fibrosis; Endothelial dysfunction; Estrogen receptors.

MeSH terms

  • Animals
  • Aorta / drug effects*
  • Aorta / pathology
  • Apoptosis / drug effects
  • Arginase / metabolism
  • Collagen Type I / metabolism
  • Endothelial Cells / drug effects*
  • Endothelial Cells / metabolism
  • Fibrosis / drug therapy*
  • Flavones / pharmacology*
  • Glucose / pharmacology*
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Immunohistochemistry
  • Insulin Resistance
  • Interleukin-1beta / metabolism
  • MAP Kinase Signaling System / drug effects
  • Male
  • NF-kappa B / metabolism
  • Nitric Oxide Synthase Type III / metabolism
  • Rats
  • Rats, Inbred SHR
  • Receptors, Estrogen / antagonists & inhibitors
  • Receptors, Estrogen / metabolism*
  • Receptors, G-Protein-Coupled / metabolism
  • Sodium Chloride / pharmacology
  • Transforming Growth Factor beta / metabolism

Substances

  • Collagen Type I
  • Flavones
  • Gper1 protein, rat
  • IL1B protein, rat
  • Interleukin-1beta
  • NF-kappa B
  • Receptors, Estrogen
  • Receptors, G-Protein-Coupled
  • Transforming Growth Factor beta
  • Sodium Chloride
  • Nitric Oxide Synthase Type III
  • Nos3 protein, rat
  • Arg2 protein, rat
  • Arginase
  • Glucose
  • acacetin