Transcriptomic analysis of peripheral blood mononuclear cells in head and neck squamous cell carcinoma patients

doi:10.1111/odi.13639

. 2021 Sep;27(6):1394-1402.

doi: 10.1111/odi.13639. Epub 2020 Sep 24.

Transcriptomic analysis of peripheral blood mononuclear cells in head and neck squamous cell carcinoma patients

Affiliations

¹ Department of Anatomy, Faculty of Medicine, Center of Excellence in Molecular Genetics of Cancer and Human Diseases, Chulalongkorn University, Bangkok, Thailand.
² Program of Medical Science, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
³ Department of Medicine, Division of Allergy and Clinical Immunology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
⁴ King Chulalongkorn Memorial Hospital, Bangkok, Thailand.
⁵ Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center- Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
⁶ Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
⁷ Department of Otolaryngology, Head and Neck Surgery, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand.
⁸ Center of Excellence in Immunology and Immune-Mediated Diseases, Chulalongkorn University, Bangkok, Thailand.
⁹ Immunology Unit, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
¹⁰ Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.

PMID: 32892371
DOI: 10.1111/odi.13639

Transcriptomic analysis of peripheral blood mononuclear cells in head and neck squamous cell carcinoma patients

Fardeela Bin-Alee et al. Oral Dis. 2021 Sep.

. 2021 Sep;27(6):1394-1402.

doi: 10.1111/odi.13639. Epub 2020 Sep 24.

Authors

Affiliations

¹ Department of Anatomy, Faculty of Medicine, Center of Excellence in Molecular Genetics of Cancer and Human Diseases, Chulalongkorn University, Bangkok, Thailand.
² Program of Medical Science, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
³ Department of Medicine, Division of Allergy and Clinical Immunology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
⁴ King Chulalongkorn Memorial Hospital, Bangkok, Thailand.
⁵ Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center- Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
⁶ Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
⁷ Department of Otolaryngology, Head and Neck Surgery, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand.
⁸ Center of Excellence in Immunology and Immune-Mediated Diseases, Chulalongkorn University, Bangkok, Thailand.
⁹ Immunology Unit, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
¹⁰ Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.

PMID: 32892371
DOI: 10.1111/odi.13639

Abstract

Objectives: To investigate the gene expression profile of peripheral blood mononuclear cells (PBMCs) from head and neck squamous cell carcinoma (HNSCC), including oral cancer (OC) and oropharyngeal cancer (OPC) patients, and compare them with healthy controls (HC).

Materials and methods: Transcriptomic analysis of PBMCs was performed by RNA-sequencing. The upregulated candidate genes were selected for validation by quantitative real-time polymerase chain reaction (qPCR). In addition, related plasma protein levels were determined by enzyme-linked immunosorbent assay (ELISA).

Results: Three significantly upregulated genes, including high mobility group nucleosomal binding domain 2 (HMGN2), folate receptor gamma (FOLR3), and amphiregulin (AREG), were selected. In the first cohort, the results showed that only HMGN2 expression was significantly increased in OC patients. In the larger sample size, the overall results demonstrated that HMGN2 expression had a tendency to increase in both OC and OPC patients compared with HC. Interestingly, the plasma HMGN2 (HMG-17) protein level exhibited the same trend as that observed at the transcriptional level.

Conclusion: HMGN2 expression and plasma HMG-17 (HMGN2 protein) were increased in both cancer patients compared with HC. This gene may be important for further functional studies in the PBMCs of HNSCC patients.

Keywords: HMGN2; RNA-sequencing; head and neck squamous cell carcinoma; peripheral blood mononuclear cells; transcriptome.

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References

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1. Arayataweegool, A., Srisuttee, R., Mahattanasakul, P., Tangjaturonsasme, N., Kerekhanjanarong, V., Kitkumthorn, N., & Mutirangura, A. (2019). Head and neck squamous cell carcinoma drives long interspersed element-1 hypomethylation in the peripheral blood mononuclear cells. Oral Diseases, 25(1), 64-72. https://doi.org/10.1111/odi.12944
1. Assaraf, Y. G., Leamon, C. P., & Reddy, J. A. (2014). The folate receptor as a rational therapeutic target for personalized cancer treatment. Drug Resistance Updates, 17(4-6), 89-95. https://doi.org/10.1016/j.drup.2014.10.002
1. Boonsongserm, P., Angsuwatcharakon, P., Puttipanyalears, C., Aporntewan, C., Kongruttanachok, N., Aksornkitti, V., … Mutirangura, A. (2019). Tumor-induced DNA methylation in the white blood cells of patients with colorectal cancer. Oncology Letters, 18(3), 3039-3048. https://doi.org/10.3892/ol.2019.10638

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[1] Amin, M. B., Greene, F. L., Edge, S. B., Compton, C. C., Gershenwald, J. E., Brookland, R. K., … Winchester, D. P. (Eds). (2017). AJCC cancer staging manual. New York City: Springer International Publishing.

[2] Amin, M. B., Greene, F. L., Edge, S. B., Compton, C. C., Gershenwald, J. E., Brookland, R. K., … Winchester, D. P. (Eds). (2017). AJCC cancer staging manual. New York City: Springer International Publishing.

[3] Andrews, S., Gilley, J., & Coleman, M. P. (2010). Difference Tracker: ImageJ plugins for fully automated analysis of multiple axonal transport parameters. Journal of neuroscience methods, 193(2), 281-287. https://doi.org/10.1016/j.jneumeth.2010.09.007

[4] Andrews, S., Gilley, J., & Coleman, M. P. (2010). Difference Tracker: ImageJ plugins for fully automated analysis of multiple axonal transport parameters. Journal of neuroscience methods, 193(2), 281-287. https://doi.org/10.1016/j.jneumeth.2010.09.007

[5] Arayataweegool, A., Srisuttee, R., Mahattanasakul, P., Tangjaturonsasme, N., Kerekhanjanarong, V., Kitkumthorn, N., & Mutirangura, A. (2019). Head and neck squamous cell carcinoma drives long interspersed element-1 hypomethylation in the peripheral blood mononuclear cells. Oral Diseases, 25(1), 64-72. https://doi.org/10.1111/odi.12944

[6] Arayataweegool, A., Srisuttee, R., Mahattanasakul, P., Tangjaturonsasme, N., Kerekhanjanarong, V., Kitkumthorn, N., & Mutirangura, A. (2019). Head and neck squamous cell carcinoma drives long interspersed element-1 hypomethylation in the peripheral blood mononuclear cells. Oral Diseases, 25(1), 64-72. https://doi.org/10.1111/odi.12944

[7] Assaraf, Y. G., Leamon, C. P., & Reddy, J. A. (2014). The folate receptor as a rational therapeutic target for personalized cancer treatment. Drug Resistance Updates, 17(4-6), 89-95. https://doi.org/10.1016/j.drup.2014.10.002

[8] Assaraf, Y. G., Leamon, C. P., & Reddy, J. A. (2014). The folate receptor as a rational therapeutic target for personalized cancer treatment. Drug Resistance Updates, 17(4-6), 89-95. https://doi.org/10.1016/j.drup.2014.10.002

[9] Boonsongserm, P., Angsuwatcharakon, P., Puttipanyalears, C., Aporntewan, C., Kongruttanachok, N., Aksornkitti, V., … Mutirangura, A. (2019). Tumor-induced DNA methylation in the white blood cells of patients with colorectal cancer. Oncology Letters, 18(3), 3039-3048. https://doi.org/10.3892/ol.2019.10638

[10] Boonsongserm, P., Angsuwatcharakon, P., Puttipanyalears, C., Aporntewan, C., Kongruttanachok, N., Aksornkitti, V., … Mutirangura, A. (2019). Tumor-induced DNA methylation in the white blood cells of patients with colorectal cancer. Oncology Letters, 18(3), 3039-3048. https://doi.org/10.3892/ol.2019.10638

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Transcriptomic analysis of peripheral blood mononuclear cells in head and neck squamous cell carcinoma patients

Affiliations

Transcriptomic analysis of peripheral blood mononuclear cells in head and neck squamous cell carcinoma patients

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