Objective: To investigate the quantitative changes of γδT cells in peripheral blood before and after anti-Brucella treatment in patients with chronic brucellosis. Methods: A prospective design was used to 88 patients with chronic brucellosis who were admitted to the Second People's Hospital of Tianjin from September 2012 to April 2018. The patients took anti-brucella drugs, And the changes in the number of γδT cell, CD3(+), CD4(+), CD8(+)T lymphocytes and CD4/8 in peripheral blood before treatment, 6 weeks and 12 weeks after treatment were analyzed. Thirty volunteers were selected as the healthy control group from Tianjin Second People's Hospital employee health checkup in 2014. Results: After 6 weeks antibacterial therapy, the counts of CD3(+), CD4(+) and CD8(+)T lymphocytes were significantly lower than before treatment in patients with chronic brucellosis (P<0.05) . After 12 weeks antibacterial therapy, the counts of γδT cell, CD3(+), CD4(+) and CD8(+)T lymphocytes were significantly lower than before treatment (P<0.05) , but CD4/8 was higher than before treatment in patients with chronic brucellosis (P<0.05) . Compared with healthy control group, after 6 weeks antibacterial treatment, the γδT cell count was still significantly higher, but the CD4(+)T lymphocyte count was lower (P<0.05) . After 12 weeks treatment, the γδT cell count was still significantly higher than that of the healthy control group (P<0.01) . Conclusion: γδ T cells, CD4(+), CD8(+) and CD3(+)T lymphocytes may play a role in human body resistance to chronic Brucella infection.
目的： 探讨γδT细胞在慢性布鲁氏菌病患者抗菌治疗前后外周血中的数量变化。 方法： 于2019年9月，选择2012年9月至2018年4月就诊于天津市第二人民医院的88例慢性布鲁氏菌病患者为研究对象，选择2014年该院职工体检健康的30人作为健康对照组。对抗布鲁氏菌治疗前、治疗后6周、12周，外周血中γδT及CD3(+)、CD4(+)、CD8(+)T淋巴细胞计数和CD4/8比值数量变化进行分析。 结果： 与治疗前比较，患者完成6周抗布鲁氏菌病治疗后CD3(+)、CD4(+)和CD8(+)T淋巴细胞计数均明显降低，差异有统计学意义（P<0.05）；患者完成12周抗布鲁氏菌治疗后γδT细胞、CD3(+)和CD8(+)T淋巴细胞计数均明显降低、CD4/8明显升高，差异均有统计学意义（P<0.05）。与健康对照组比较，患者治疗前γδT细胞、CD8(+)和CD3(+)T淋巴细胞计数较高、CD4/8计数较低，差异均有统计学意义（P<0.05）；患者完成6周抗布鲁氏菌病治疗后，γδT细胞计数较高、CD4(+)T淋巴细胞计数较低，差异均有统计学意义（P<0.05）；患者完成12周抗布鲁氏菌病治疗后γδT细胞计数较高，差异有统计学意义（P<0.01）。 结论： γδT细胞、CD4(+)、CD8(+)和CD3(+)T淋巴细胞可能在机体抵制布鲁氏菌慢性感染中发挥作用。.
Keywords: Brucellosis; CD3(+) T lymphocytes; CD4(+)T lymphocytes; CD8(+)T lymphocytes; γδT cells.