This site needs JavaScript to work properly. Please enable it to take advantage of the complete set of features!

Skip to main page content

Email citation

Add to Collections

Your saved search

Name of saved search:

Search terms:

Test search terms

Would you like email updates of new search results?

Yes
No

Email: (change)

Frequency:

Which day?

Which day?

Report format:

Send at most:

Send even when there aren't any new results

Optional text in email:

Your RSS Feed

. 2020 Sep;585(7824):313-314.

doi: 10.1038/d41586-020-02549-1.

When antibodies mislead: the quest for validation

PMID: 32895531
DOI: 10.1038/d41586-020-02549-1

When antibodies mislead: the quest for validation

Monya Baker. Nature. 2020 Sep.

. 2020 Sep;585(7824):313-314.

doi: 10.1038/d41586-020-02549-1.

Author

PMID: 32895531
DOI: 10.1038/d41586-020-02549-1

No abstract available

Keywords: Biological techniques; Research management.

PubMed Disclaimer

Comment in

Commercial antibodies: alternative grading for research.
Blackshaw S, Zhu H. Blackshaw S, et al. Nature. 2020 Oct;586(7830):500. doi: 10.1038/d41586-020-02929-7. Nature. 2020. PMID: 33082538 No abstract available.

Similar articles

Reproducibility crisis: Blame it on the antibodies.
Baker M. Baker M. Nature. 2015 May 21;521(7552):274-6. doi: 10.1038/521274a. Nature. 2015. PMID: 25993940 No abstract available.
Although it's painful: The importance of stringent antibody validation.
Meliopoulos VA, Schultz-Cherry S. Meliopoulos VA, et al. PLoS Pathog. 2018 Jan 4;14(1):e1006701. doi: 10.1371/journal.ppat.1006701. eCollection 2018 Jan. PLoS Pathog. 2018. PMID: 29300781 Free PMC article. No abstract available.
Antibody anarchy: A call to order.
Baker M. Baker M. Nature. 2015 Nov 26;527(7579):545-51. doi: 10.1038/527545a. Nature. 2015. PMID: 26607547 No abstract available.
Antibodies That Work Again and Again and Again.
Simpson K, Browning M. Simpson K, et al. Methods Mol Biol. 2017;1554:41-59. doi: 10.1007/978-1-4939-6759-9_2. Methods Mol Biol. 2017. PMID: 28185182 Review.
Antibody validation: a view from the mountains.
Taussig MJ, Fonseca C, Trimmer JS. Taussig MJ, et al. N Biotechnol. 2018 Oct 25;45:1-8. doi: 10.1016/j.nbt.2018.08.002. Epub 2018 Aug 4. N Biotechnol. 2018. PMID: 30086383 Free PMC article. Review.

See all similar articles

Cited by

The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata.
Kamata K, Mizutani K, Takahashi K, Marchetti R, Silipo A, Addy C, Park SY, Fujii Y, Fujita H, Konuma T, Ikegami T, Ozeki Y, Tame JRH. Kamata K, et al. Sci Rep. 2020 Dec 16;10(1):22102. doi: 10.1038/s41598-020-78926-7. Sci Rep. 2020. PMID: 33328520 Free PMC article.
The Power of Reagent Titration in Flow Cytometry.
Bonilla DL, Paul A, Gil-Pulido J, Park LM, Jaimes MC. Bonilla DL, et al. Cells. 2024 Oct 11;13(20):1677. doi: 10.3390/cells13201677. Cells. 2024. PMID: 39451195 Free PMC article. Review.
A commercial ARHGEF17/TEM4 antibody cross-reacts with Nuclear Mitotic Apparatus protein 1 (NuMA).
Prifti DK, Lauzier A, Elowe S. Prifti DK, et al. PLoS One. 2022 Jul 1;17(7):e0268848. doi: 10.1371/journal.pone.0268848. eCollection 2022. PLoS One. 2022. PMID: 35776709 Free PMC article.
Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining.
Hurd PJ, Grübel K, Wojciechowski M, Maleszka R, Rössler W. Hurd PJ, et al. Sci Rep. 2021 Mar 25;11(1):6852. doi: 10.1038/s41598-021-86078-5. Sci Rep. 2021. PMID: 33767244 Free PMC article.
Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications.
Ayoubi R, Ryan J, Biddle MS, Alshafie W, Fotouhi M, Bolivar SG, Ruiz Moleon V, Eckmann P, Worrall D, McDowell I, Southern K, Reintsch W, Durcan TM, Brown C, Bandrowski A, Virk H, Edwards AM, McPherson P, Laflamme C. Ayoubi R, et al. Elife. 2023 Nov 23;12:RP91645. doi: 10.7554/eLife.91645. Elife. 2023. PMID: 37995198 Free PMC article.

See all "Cited by" articles

References

1. Laflamme, C. et al. eLife 8, e48363 (2019). - PubMed - DOI - PMC
1. Andersson, S. et al. Nature Commun. 8, 15840 (2017). - PubMed - DOI
1. Voskuil, J. L. A. et al. mAbs 12, 1794421 (2020). - PubMed - DOI
1. Uhlen, M. et al. Nature Meth. 13, 823–827 (2016). - DOI
1. Menke, J., Roelandse, M., Ozyurt, B., Martone, M. & Bandrowski, A. Preprint at bioRxiv https://doi.org/10.1101/2020.01.15.908111 (2020).

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions

LinkOut - more resources

Full Text Sources
- Nature Publishing Group