The emergence of hypervirulent blaNDM-1-positive Klebsiella pneumoniae sequence type 395 in an oncology hospital

Irina Lazareva; Vladimir Ageevets; Julia Sopova; Marina Lebedeva; Polina Starkova; Daria Likholetova; Maria Lebedeva; Vladimir Gostev; Vladimir Moiseenko; Vitaliy Egorenkov; Arina Navatskaya; Galina Mitroshina; Elena Myasnikova; Irina Tsvetkova; Yuri Lobzin; Sergey Sidorenko

doi:10.1016/j.meegid.2020.104527

The emergence of hypervirulent bla_NDM-1-positive Klebsiella pneumoniae sequence type 395 in an oncology hospital

Infect Genet Evol. 2020 Nov:85:104527. doi: 10.1016/j.meegid.2020.104527. Epub 2020 Sep 6.

Authors

Irina Lazareva¹, Vladimir Ageevets¹, Julia Sopova², Marina Lebedeva³, Polina Starkova⁴, Daria Likholetova⁵, Maria Lebedeva⁶, Vladimir Gostev⁷, Vladimir Moiseenko³, Vitaliy Egorenkov³, Arina Navatskaya³, Galina Mitroshina³, Elena Myasnikova⁸, Irina Tsvetkova¹, Yuri Lobzin⁷, Sergey Sidorenko⁹

Affiliations

¹ Pediatric Research and Clinical Center for Infectious Diseases, Professor Popov Str. 9, Saint Petersburg 197022, Russia.
² Saint Petersburg University, Universitetskaya Emb. 13B, Saint Petersburg 199034, Russia.
³ Clinical Research and Practical Center of Specialized Types of Medical Care (Oncologic), Posolok Pesochnyy, Leningradskaya Str. 68А, Saint Petersburg 197758, Russia.
⁴ National Research Institute of Information Technologies, Mechanics and Optics, Lomonosova Str. 9, Saint Petersburg 191002, Russia.
⁵ Pediatric Research and Clinical Center for Infectious Diseases, Professor Popov Str. 9, Saint Petersburg 197022, Russia; Saint Petersburg University, Universitetskaya Emb. 13B, Saint Petersburg 199034, Russia.
⁶ Bryansk Interregional Veterinary Laboratory, Suponevo, Shosseynaya Str. 7, Bryansk 241520, Russia.
⁷ Pediatric Research and Clinical Center for Infectious Diseases, Professor Popov Str. 9, Saint Petersburg 197022, Russia; North-Western State Medical University named after I.I. Mechnikov, Kirochnaya street, 41, Saint Petersburg 191015, Russia.
⁸ Clinical Research and Practical Center of Specialized Types of Medical Care (Oncologic), Posolok Pesochnyy, Leningradskaya Str. 68А, Saint Petersburg 197758, Russia; North-Western State Medical University named after I.I. Mechnikov, Kirochnaya street, 41, Saint Petersburg 191015, Russia.
⁹ Pediatric Research and Clinical Center for Infectious Diseases, Professor Popov Str. 9, Saint Petersburg 197022, Russia; North-Western State Medical University named after I.I. Mechnikov, Kirochnaya street, 41, Saint Petersburg 191015, Russia. Electronic address: sidorserg@niidi.ru.

PMID: 32898687
DOI: 10.1016/j.meegid.2020.104527

Abstract

Fifteen hypermucoviscous isolates (13 bla_NDM-1-positive) obtained from 11 oncology patients were analyzed by whole genome sequencing, and selected isolates were assessed in a murine model of sepsis. ST395/K2 isolates harboring rmpA, rmpA2, peg-344, aerobactin, enterobactin, yersiniabactin, type I fimbriae, etc. displayed maximal virulence in the mouse lethality assay (LD₅₀ = 10² CFU). ST147/K20 isolates lacking yersiniabactins were relatively less virulent (LD₅₀ = 10⁴ CFU), ST395/K2 isolates lacking rmpA, rmpA2, peg-344, and aerobactin, but harboring yersiniabactin demonstrated minimal virulence (LD₅₀ = 10⁵ CFU). Isolates represent various paths and stages of evolution directed towards convergence of multidrugresistant classical Klebsiella pneumoniae and hypervirulent K. pneumoniae.

Keywords: Antimicrobial resistance; Hypermucoviscous Klebsiella pneumoniae; New Delhi metallo-beta-lactamase 1; Virulence.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / therapeutic use*
Drug Resistance, Bacterial / genetics*
Humans
Klebsiella Infections / drug therapy*
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / pathogenicity*
Mice
Microbial Sensitivity Tests
Models, Animal
Multilocus Sequence Typing
Sepsis / genetics
Sepsis / microbiology
Virulence / genetics*
Whole Genome Sequencing
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
beta-Lactamases
beta-lactamase NDM-1