Highly sensitive determination of amanita toxins in biological samples using β-cyclodextrin collaborated molecularly imprinted polymers coupled with ultra-high performance liquid chromatography tandem mass spectrometry

Lei Tan; Yongxian Li; Fenfang Deng; Xinhong Pan; Hong Yu; María Luisa Marina; Zhengjin Jiang

doi:10.1016/j.chroma.2020.461514

Highly sensitive determination of amanita toxins in biological samples using β-cyclodextrin collaborated molecularly imprinted polymers coupled with ultra-high performance liquid chromatography tandem mass spectrometry

J Chromatogr A. 2020 Aug 31:1630:461514. doi: 10.1016/j.chroma.2020.461514. Online ahead of print.

Authors

Lei Tan¹, Yongxian Li², Fenfang Deng², Xinhong Pan², Hong Yu², María Luisa Marina³, Zhengjin Jiang⁴

Affiliations

¹ Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China; Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares, Madrid, Spain; Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China.
² Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
³ Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares, Madrid, Spain. Electronic address: mluisa.marina@uah.es.
⁴ Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China. Electronic address: jzjjackson@hotmail.com.

PMID: 32898756
DOI: 10.1016/j.chroma.2020.461514

Abstract

In this work, a β-cyclodextrin functional vinyl monomer was synthesized and the common moiety of five amanita toxins was used as the template for preparing molecularly imprinted polymers (MIPs). Chemical calculation was used to evaluate and describe the binding interactions between the template and the functional monomer. The preparation conditions were optimized and the resultant MIPs were characterized and employed as solid-phase extraction (SPE) sorbents. The SPE conditions including the amount of sorbent, extraction solution, and eluting solution were also optimized for the enrichment of the five toxins. Using an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), detection limits ranging from 0.34-0.42 µg/L, 0.16-0.33 µg/L, and 0.035-0.056 µg/kg were achieved for the five toxins in serum, urine and liver samples, respectively. The proposed method was further applied to the determination of the amanita toxins in suspected samples and showed great potential in the diagnosis of mushroom poisoning.

Keywords: Amanita toxins; Molecularly imprinted polymers; Mushroom poisoning; Solid phase extraction; β-cyclodextrin.