In vitro bioactivity, antimicrobial and anti-inflammatory efficacy of modified solvent evaporation assisted Trametes versicolor extract

3 Biotech. 2020 Sep;10(9):404. doi: 10.1007/s13205-020-02397-w. Epub 2020 Aug 25.

Abstract

In the present study, modified solvent evaporation assisted methanolic Trametes versicolor extract was evaluated for its antimicrobial and anti-inflammatory efficacy. Mushroom extract showed significantly (p < 0.05) higher total phenolic content (48.71 mg/g) followed by total flavonoid content (13.13 mg/g), ascorbic acid content (11.03 mg/g), β-carotene content (8.34), and lycopene content (6.85). Fourier transform infrared spectroscopy revealed the functional groups of the observed bioactive compounds, while HPLC chromatogram showed significantly (p < 0.05) higher gallic acid content (45.72 mg/g) as compared to rutin content (12.50 mg/g). Statistically, mushroom extract and artificial antioxidant (BHA) showed a non-significant (p < 0.05) difference in terms of percentage inhibition during DPPH and N2O2 scavenging assay. During the time-kill kinetics, the mushroom extract significantly inhibited the growth of S. aureus in comparison with the growth of P.aeruginosa, K. pneumonia, and E.coli. Mushroom extract showed effective anti-inflammatory activity during membrane stabilization (33.71-73.24%) and protein denaturation (23.11-74.56%) assays.

Keywords: Anti-inflammatory; FTIR; HPLC; Mushroom; Trametes versicolor.