NUP62 is required for the maintenance of the spindle assembly checkpoint and chromosomal stability

Int J Biochem Cell Biol. 2020 Nov:128:105843. doi: 10.1016/j.biocel.2020.105843. Epub 2020 Sep 6.


The nuclear pore protein NUP62 localizes to spindle poles in mitosis and plays a role in maintaining centrosome homeostasis. In this study, we found that NUP62-depleted cells exhibited a defective spindle assembly checkpoint (SAC) and that depletion of NUP62 caused a slight decrease in MAD2 protein levels after nocodazole treatment. However, depletion of NUP62 did not cause a failure in kinetochore localization of the SAC proteins BUBR1, MAD1, and MAD2 in prometaphase. NUP62 depletion slightly prolonged mitotic timing but did not affect cell doubling time. In addition, NUP62 depletion caused a SAC defect and induced aneuploidy in human neural stem cells. Furthermore, overexpression of NUP62Q391P, a mutant protein causing autosomal recessive infantile bilateral striatal necrosis, resulted in a defect in the SAC, indicating that the amino acid residue Q391 in NUP62 is crucial for its effect on the SAC. Overall, we conclude that NUP62 maintains the SAC downstream of kinetochores and thereby ensures maintenance of chromosomal stability.

Keywords: Chromosomal stability; NUP62; Spindle assembly checkpoint.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Cell Cycle Checkpoints*
  • Chromosomal Instability*
  • Chromosomes, Human / genetics
  • Chromosomes, Human / metabolism*
  • HCT116 Cells
  • HeLa Cells
  • Humans
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism*
  • Mutation, Missense
  • Nuclear Pore Complex Proteins / genetics
  • Nuclear Pore Complex Proteins / metabolism*
  • Spindle Apparatus / genetics
  • Spindle Apparatus / metabolism*


  • Membrane Glycoproteins
  • Nuclear Pore Complex Proteins
  • nuclear pore protein p62