Sepiapterin reductase in human amniotic and skin fibroblasts, chorionic villi, and various blood fractions

Clin Chim Acta. 1988 Jun 15;174(3):271-82. doi: 10.1016/0009-8981(88)90053-8.

Abstract

Sepiapterin reductase activity has been measured in amniotic fibroblasts by two procedures: one photometric and the other HPLC-fluorimetric. Both can be used for quantitative measurements, but the latter has considerable advantages including smaller standard deviation, much lower detection limit, and less volume of sample required. Sepiapterin reductase activity was also assayed in skin fibroblasts, chorionic villi and various blood fractions including stimulated mononuclear blood cells. Red blood cells have a low specific activity compared to unstimulated mononuclear blood cells, although the latter have a mean value with a high standard deviation. When the mononuclear blood cells were cultured for 5 days, the mean specific activity increased and the range became tighter. Enzyme stability and N-acetylserotonin inhibition were also studied.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alcohol Oxidoreductases / analysis*
  • Alcohol Oxidoreductases / antagonists & inhibitors
  • Alcohol Oxidoreductases / blood
  • Amnion / enzymology
  • Chorionic Villi / enzymology
  • Chromatography, High Pressure Liquid
  • Enzyme Stability
  • Erythrocytes / enzymology
  • Fibroblasts / enzymology*
  • Humans
  • Leukocytes, Mononuclear / enzymology
  • Serotonin / analogs & derivatives
  • Serotonin / pharmacology
  • Skin / enzymology
  • Spectrometry, Fluorescence
  • Spectrophotometry / methods

Substances

  • Serotonin
  • Alcohol Oxidoreductases
  • sepiapterin reductase
  • N-acetylserotonin