Effect of isgin ( Rheum ribes L.) on biochemical parameters, antioxidant activity and DNA damage in rats with obesity induced with high-calorie diet

Arch Physiol Biochem. 2020 Sep 12;1-9. doi: 10.1080/13813455.2020.1819338. Online ahead of print.

Abstract

The present study was designed to investigate the effects of Rheum ribes L. plant root extracts on DNA damage, biochemical and antioxidant parameters in rats with experimental obesity induced with a high-calorie diet. The study groups were divided as "normal control(NC)", "obese control(OC)", "obese + Rheum ribes(OR1)(200 mg/kg)" and "obese + Rheum ribes (OR2)(400 mg/kg)". At the end of the application, rats were sacrificed and blood and tissue samples were obtained. According to the results obtained, the marker of DNA damage in tissues of 8-OHdG was determined to be significantly reduced in brain tissue of the OR1 and OR2 groups compared to the NC group. However, fluctuations were identified in the MDA activity, antioxidant defense system elements and serum biomarkers in tissues. In conclusion, Rheum ribes plant root extract ensured improvements in DNA damage in brain tissues and MDA levels and showed positive effects on antioxidant parameter activities in different tissues.

Keywords: Antiobesity; DNA damage; Rheum ribes L; antioxidant activity.