Plasma pharmacokinetics of (poly)phenol metabolites and catabolites after ingestion of orange juice by endurance trained men

Gema Pereira-Caro; Michael N Clifford; Thelma Polyviou; Iziar A Ludwig; Hani Alfheeaid; José Manuel Moreno-Rojas; Ada L Garcia; Dalia Malkova; Alan Crozier

doi:10.1016/j.freeradbiomed.2020.09.007

Plasma pharmacokinetics of (poly)phenol metabolites and catabolites after ingestion of orange juice by endurance trained men

Free Radic Biol Med. 2020 Nov 20:160:784-795. doi: 10.1016/j.freeradbiomed.2020.09.007. Epub 2020 Sep 11.

Authors

Gema Pereira-Caro¹, Michael N Clifford², Thelma Polyviou³, Iziar A Ludwig⁴, Hani Alfheeaid⁵, José Manuel Moreno-Rojas⁶, Ada L Garcia³, Dalia Malkova³, Alan Crozier⁷

Affiliations

¹ Department of Food and Health, Andalusian Institute of Agricultural and Fishery Research and Training, IFAPA, Alameda Del Obispo, 14004, Córdoba, Spain. Electronic address: mariag.pereira@juntadeandalucia.es.
² School of Bioscience and Medicine, Faculty of Health and Medical Sciences University of Surrey, Guildford, GU2 5XH, United Kingdom; Department of Nutrition, Dietetics and Food, Monash University, Notting Hill, VIC, 3168, Australia.
³ Human Nutrition, New Lister Building, University of Glasgow, Glasgow Royal Infirmary, 10-16 Alexandra Parade, Glasgow, G31 2ER, UK.
⁴ Medicinal Chemistry Laboratory, Center for Applied Medicinal Research, University of Navarra, Avda. Pío XII 55, E-31008, Pamplona, Spain.
⁵ Department of Food Science and Human Nutrition, College of Agriculture and Veterinary Medicine, Qassim University, Saudi Arabia.
⁶ Department of Food and Health, Andalusian Institute of Agricultural and Fishery Research and Training, IFAPA, Alameda Del Obispo, 14004, Córdoba, Spain.
⁷ School of Medicine, Dentistry and Nursing, University of Glasgow, Glasgow, G12 8QQ, UK; United Kingdom and Department of Nutrition, University of California, Davis, CA, 95616, USA.

PMID: 32927016
DOI: 10.1016/j.freeradbiomed.2020.09.007

Abstract

The health benefits of orange juice (OJ) consumption are attributed in part to the circulating flavanone phase II metabolites and their microbial-derived ring fission phenolic catabolites. The present study investigated these compounds in the bloodstream after acute intake of 500 mL of OJ. Plasma samples obtained at 0, 1, 2, 3, 4, 5, 6, 7, 8 and 24 h after OJ intake were analysed by HPLC-HR-MS. Eleven flavanone metabolites and 36 phenolic catabolites were identified and quantified in plasma. The main metabolites were hesperetin-3'-sulfate with a peak plasma concentration (C_max) of 80 nmol/L, followed by hesperetin-7-glucuronide (C_max 24 nmol/L), hesperetin-3'-glucuronide (C_max 18 nmol/L) and naringenin-7-glucuronide (C_max 21 nmol/L). Among the main phenolic catabolites to increase in plasma after OJ consumption were 3'-methoxycinnamic acid-4'-sulfate (C_max 19 nmol/L), 3-hydroxy-3-(3'-hydroxy-4'-methoxyphenyl)propanoic acid (C_max 20 nmol/L), 3-(3'-hydroxy-4'-methoxyphenyl)propanoic acid (C_max 19 nmol/L), 3-(4'-hydroxyphenyl)propanoic acid (C_max 25 nmol/L), and 3-(phenyl)propanoic acid (C_max 19 nmol/L), as well as substantial amounts of phenylacetic and hippuric acids. The comprehensive plasma pharmacokinetic profiles that were obtained are of value to the design of future ex vivo cell studies, aimed at elucidating the mechanisms underlying the potential health benefits of OJ consumption. CLINICAL TRIAL REGISTRATION NUMBER: This trial was registered at clinicaltrials.gov as NCT02627547.

Keywords: 3-Hydroxy-3-(phenyl)propanoic acids; Absorption; Metabolites; Microbial catabolites; Orange juice (poly)phenols; Potential bioactivity.

Publication types

Clinical Trial, Phase II
Research Support, Non-U.S. Gov't

MeSH terms

Citrus sinensis*
Eating
Fruit and Vegetable Juices
Humans
Male
Phenol
Phenols

Substances

Phenols
Phenol

Associated data

ClinicalTrials.gov/NCT02627547