Structural Evidence for a [4Fe-5S] Intermediate in the Non-Redox Desulfuration of Thiouracil

Angew Chem Int Ed Engl. 2021 Jan 4;60(1):424-431. doi: 10.1002/anie.202011211. Epub 2020 Nov 4.


We recently discovered a [Fe-S]-containing protein with in vivo thiouracil desulfidase activity, dubbed TudS. The crystal structure of TudS refined at 1.5 Å resolution is reported; it harbors a [4Fe-4S] cluster bound by three cysteines only. Incubation of TudS crystals with 4-thiouracil trapped the cluster with a hydrosulfide ligand bound to the fourth non-protein-bonded iron, as established by the sulfur anomalous signal. This indicates that a [4Fe-5S] state of the cluster is a catalytic intermediate in the desulfuration reaction. Structural data and site-directed mutagenesis indicate that a water molecule is located next to the hydrosulfide ligand and to two catalytically important residues, Ser101 and Glu45. This information, together with modeling studies allow us to propose a mechanism for the unprecedented non-redox enzymatic desulfuration of thiouracil, in which a [4Fe-4S] cluster binds and activates the sulfur atom of the substrate.

Keywords: [4Fe-5S] clusters; cluster compounds; desulfidase; sulfuration; thiouracil.

Publication types

  • Research Support, Non-U.S. Gov't