Identification of two integral membrane proteins of Plasmodium falciparum

Proc Natl Acad Sci U S A. 1988 Jul;85(14):5195-9. doi: 10.1073/pnas.85.14.5195.

Abstract

We describe the isolation and cloning of two integral membrane protein antigens of Plasmodium falciparum. The antigens were isolated by Triton X-114 temperature-dependent phase separation, electrophoretically transferred to nitrocellulose, and used to affinity-purify monospecific human antibodies. These antibodies were used to isolate the corresponding cDNA clones from a phage lambda gt11-Amp3 cDNA expression library. Clone Ag512 corresponds to a Mr 55,000 merozoite rhoptry antigen, and clone Ag513 corresponds to a Mr 45,000 merozoite surface antigen. Both proteins can be biosynthetically labeled with [3H]glucosamine and [3H]myristic acid, suggesting that they may be anchored in membranes via a glycosylphosphatidylinositol moiety. Similarities in the C-terminal sequences of the Mr 45,000 merozoite surface antigen and the Trypanosoma brucei variant surface glycoproteins provides further evidence that this antigen has a glycosylphosphatidylinositol anchor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Protozoan / isolation & purification
  • Antigens, Protozoan / analysis*
  • Antigens, Protozoan / genetics
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • DNA / isolation & purification
  • DNA, Recombinant / isolation & purification
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / genetics
  • Fluorescent Antibody Technique
  • Humans
  • Immunoassay
  • Immunosorbent Techniques
  • Membrane Glycoproteins*
  • Membrane Proteins / analysis*
  • Membrane Proteins / genetics
  • Membrane Proteins / immunology
  • Molecular Sequence Data
  • Molecular Weight
  • Phosphatidylinositols / immunology
  • Plasmodium falciparum / immunology*
  • Protozoan Proteins*
  • Sequence Homology, Nucleic Acid

Substances

  • Antibodies, Protozoan
  • Antigens, Protozoan
  • DNA, Recombinant
  • GYMSSA antigen, Plasmodium falciparum
  • Membrane Glycoproteins
  • Membrane Proteins
  • Phosphatidylinositols
  • Protozoan Proteins
  • DNA

Associated data

  • GENBANK/J03828