Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells

Cecile Davaine; Eva Hadadi; William Taylor; Annelise Bennaceur-Griscelli; Hervé Acloque

doi:10.1007/978-1-0716-0779-4_26

Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells

Methods Mol Biol. 2021:2179:341-351. doi: 10.1007/978-1-0716-0779-4_26.

Authors

Cecile Davaine¹, Eva Hadadi¹, William Taylor¹, Annelise Bennaceur-Griscelli^{2

3

4}, Hervé Acloque^{5

6}

Affiliations

¹ Inserm, UMRS935 ESTeam Paris Sud, Malignant and Therapeutic Stem Cell Models, Villejuif, France.
² Inserm, UMRS935 ESTeam Paris Sud, Malignant and Therapeutic Stem Cell Models, Villejuif, France. annelise.bennaceur@inserm.fr.
³ Service d'hématologie, APHP, GHU Paris Sud, Villejuif, France. annelise.bennaceur@inserm.fr.
⁴ University Paris Sud, University Paris-Saclay, UFR de Médecine Kremlin Bicêtre, Le Kremlin-Bicêtre, France. annelise.bennaceur@inserm.fr.
⁵ Inserm, UMRS935 ESTeam Paris Sud, Malignant and Therapeutic Stem Cell Models, Villejuif, France. herve.acloque@inra.fr.
⁶ GABI, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France. herve.acloque@inra.fr.

PMID: 32939732
DOI: 10.1007/978-1-0716-0779-4_26

Abstract

Epithelial-Mesenchymal Transition (EMT) and its reciprocal Mesenchymal-Epithelial Transition (MET) occur naturally as a cycling process during embryonic and foetal development. The capacity of such iterative cycles to drive cell fate and cellular and molecular behaviour in physiology or pathology remains unclear. We describe here a protocol to induce successive cycles of EMT/MET in an untransformed human mammary epithelial cell line (MCF10A) as well as the necessary controls for cycle validation.

Keywords: Cycles; EMT; Epithelial-mesenchymal transition; MCF10A; MET; Mesenchymal-epithelial transition.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cellular Reprogramming Techniques / methods*
Epithelial Cells / cytology*
Epithelial Cells / metabolism
Epithelial-Mesenchymal Transition*
Humans
Mammary Glands, Human / cytology*