Expression of the FGF-related proto-oncogene int-2 during gastrulation and neurulation in the mouse

EMBO J. 1988 Mar;7(3):691-5.


The proto-oncogene int-2 has been implicated in the formation of mouse mammary-tumour-virus-induced mammary tumours. Analysis of the predicted coding sequence indicates that int-2 is a member of the fibroblast growth factor family. Previous studies using Northern blot analysis suggested that normal expression of int-2 may be confined to extra-embryonic endoderm lineages of embryonic stages of mouse development. We have used in situ hybridization and Northern blot analysis to examine directly int-2 expression in embryo stem cells and in the developing embryo from early gastrulation to midsomite stages. Complex patterns of accumulation of int-2 RNA were observed in embryonic and extra-embryonic tissues. The data suggest multiple roles for int-2 in development which may include migration of early mesoderm cells and induction of the otocyst.

MeSH terms

  • Animals
  • Brain / embryology
  • Brain / metabolism
  • Embryo, Mammalian / metabolism*
  • Endoderm / metabolism
  • Fibroblast Growth Factors / physiology*
  • Gene Expression Regulation*
  • Mesoderm / metabolism
  • Mice
  • Mice, Inbred CBA
  • Nucleic Acid Hybridization
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins / physiology
  • RNA, Messenger / metabolism*
  • RNA, Messenger / physiology


  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Fibroblast Growth Factors