Eukaryotic cell biology is temporally coordinated to support the energetic demands of protein homeostasis

Nat Commun. 2020 Sep 17;11(1):4706. doi: 10.1038/s41467-020-18330-x.

Abstract

Yeast physiology is temporally regulated, this becomes apparent under nutrient-limited conditions and results in respiratory oscillations (YROs). YROs share features with circadian rhythms and interact with, but are independent of, the cell division cycle. Here, we show that YROs minimise energy expenditure by restricting protein synthesis until sufficient resources are stored, while maintaining osmotic homeostasis and protein quality control. Although nutrient supply is constant, cells sequester and store metabolic resources via increased transport, autophagy and biomolecular condensation. Replete stores trigger increased H+ export which stimulates TORC1 and liberates proteasomes, ribosomes, chaperones and metabolic enzymes from non-membrane bound compartments. This facilitates translational bursting, liquidation of storage carbohydrates, increased ATP turnover, and the export of osmolytes. We propose that dynamic regulation of ion transport and metabolic plasticity are required to maintain osmotic and protein homeostasis during remodelling of eukaryotic proteomes, and that bioenergetic constraints selected for temporal organisation that promotes oscillatory behaviour.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autophagy / physiology
  • Bioreactors
  • Circadian Rhythm
  • Energy Metabolism / physiology*
  • Eukaryotic Cells / physiology*
  • Glycogen / metabolism
  • Heat-Shock Response
  • Ionomycin
  • Mechanistic Target of Rapamycin Complex 1 / metabolism
  • Metabolomics
  • Molecular Chaperones
  • Osmolar Concentration
  • Osmotic Pressure
  • Oxygen / metabolism
  • Protein Biosynthesis
  • Protein Processing, Post-Translational
  • Proteome
  • Proteomics
  • Proteostasis / physiology*
  • Ribosomes
  • Yeasts / physiology

Substances

  • Molecular Chaperones
  • Proteome
  • Ionomycin
  • Glycogen
  • Mechanistic Target of Rapamycin Complex 1
  • Oxygen