ACC deaminase and antioxidant enzymes producing halophilic Enterobacter sp. PR14 promotes the growth of rice and millets under salinity stress

A Sagar; R Z Sayyed; P W Ramteke; S Sharma; Najat Marraiki; Abdallah M Elgorban; Asad Syed

doi:10.1007/s12298-020-00852-9

ACC deaminase and antioxidant enzymes producing halophilic Enterobacter sp. PR14 promotes the growth of rice and millets under salinity stress

Physiol Mol Biol Plants. 2020 Sep;26(9):1847-1854. doi: 10.1007/s12298-020-00852-9. Epub 2020 Aug 9.

Authors

A Sagar^{1

2}, R Z Sayyed³, P W Ramteke⁴, S Sharma¹, Najat Marraiki⁵, Abdallah M Elgorban^{5

6}, Asad Syed⁵

Affiliations

¹ Department of Microbiology and Biotechnology, Meerut Institute of Engineering and Technology, Meerut, Uttar Pradesh India.
² Department of Industrial Microbiology, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, UP 211007 India.
³ Department of Microbiology, PSGVP Mandal's, Arts, Science and Commerce College, Shahada, Maharashtra 425 409 India.
⁴ Department of Biological Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, UP 211007 India.
⁵ Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451 Saudi Arabia.
⁶ Center of Excellence in Biotechnology Research, King Saud University, P.O Box 2455, Riyadh, Saudi Arabia.

Abstract

Rhizobacteria are known to ameliorate salinity stress through a wide variety of mechanisms including the production of aminocyclopropane-1-carboxylate deaminase (ACCD). Application of ACCD positive halophilic rhizobacteria ameliorate soil salinity along with its plant growth promotion activity. An effect of the inoculation of ACCD and antioxidant positive and halophilic Enterobacter sp. PR14 was reported on the seed germination and growth of rice and millet seedlings grown in saline and alkaline soil was evaluated. The rhizobacterial strain grew well over a high level of NaCl (15-90 M); at a wide range of pH (5-9); and produced a wide variety of plant growth-promoting (PGP) traits viz. indole-acetic acid (13 µg mL^-1), ACCD (5.20 M mg^-1 h^-1), phosphate solubilization (0.99 g mL^-1) and antioxidant enzymes such as superoxide dismutase (5.143 IU mg^-1 protein), catalase (0.43 IU mg^-1 protein) and glutathione (19.077 µg mg^-1 protein) during log phase (30 h) of its growth. The stress with alkaline pH (9) and high salinity (90 M) caused a further increase in the synthesis of PGP traits, ACCD, and antioxidant enzymes. The combined application of Enterobacter sp. PR14, ammonium sulfate (as a substitute of ACC), and NaCl (30 M) resulted in a further increase in the seed germination and vigor in rice and millets vis-à-vis control and other treatments. After 15 days of growth, 61.72% more seed germination in rice and millet and 63.15% increase in sorghum was recorded over the control, and after 30 days of growth, 99.67%, 30%, and 54%, root length 50%, 30% and 54% shoot length in rice, sorghum and millet were observed respectively. A significant increase of 38.13%, 30.75%, and 16.36% in dry weight of rice, sorghum, and millet shoots was recorded respectively. Enterobacter sp PR 14, showing multiple plant growth-promoting traits has a great potential to be used as an efficient bioinoculant for growth promotion of rice and millets under alkaline and saline conditions.

Keywords: Alkalinity; Ammonium sulfate; Enterobacter sp.; Finger millet; Growth promotion; PGP traits; Rice; Salinity; Sorghum.