Transcriptomic data on the transgenerational exposure of the keystone amphipod Gammarus locusta to simvastatin

Teresa Neuparth; André M Machado; Rosa Montes; Rosario Rodil; Susana Barros; Nélson Alves; Raquel Ruivo; Luis Filipe C Castro; José B Quintana; Miguel M Santos

doi:10.1016/j.dib.2020.106248

Transcriptomic data on the transgenerational exposure of the keystone amphipod Gammarus locusta to simvastatin

Data Brief. 2020 Aug 31:32:106248. doi: 10.1016/j.dib.2020.106248. eCollection 2020 Oct.

Authors

Teresa Neuparth¹, André M Machado^{1

2}, Rosa Montes³, Rosario Rodil³, Susana Barros¹, Nélson Alves¹, Raquel Ruivo¹, Luis Filipe C Castro^{1

2}, José B Quintana³, Miguel M Santos^{1

2}

Affiliations

¹ CIMAR/CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, Portugal.
² FCUP - Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal.
³ Department of Analytical Chemistry, Nutrition and Food Sciences, IAQBUS - Institute of Research on Chemical and Biological Analysis, Universidade de Santiago de Compostela, R. Constantino Candeira S/N, 15782 Santiago de Compostela, Spain.

Abstract

The use of transcriptomics data brings new insights and works as a powerful tool to explore the molecular mode of action (MoA) of transgenerational inheritance effects of contaminants of emerging concern. Therefore, in this dataset, we present the transcriptomic data of the transgenerational effects of environmentally relevant simvastatin levels, one of the most prescribed human pharmaceuticals, in the keystone amphipod species Gammarus locusta. In summary, G. locusta juveniles were maintained under simvastatin exposure up to adulthood (exposed group - F0E) and the offspring of F0E were transferred to control water for the three subsequent generations (transgenerational group - F1T, F2T and F3T). To gain insights into the biological functions and canonical pathways transgenerationally disrupted by simvastatin, a G. locusta de novo transcriptome assembly was produced and the transcriptomic profiles of three individual G. locusta females, per group, over the four generations (F0 to F3) - solvent control groups (F0.C, F1.C, F2.C and F3.C), F0 320 ng/L simvastatin exposed group (F0.320E) and F1 to F3 320 transgenerational group (F1.320T; F2.320T and F3.320T) - were analyzed. Briefly, Illumina HiSeq™ 2500 platform was used to perform RNA sequencing, and due to the unavailability of G. locusta genome, the RNA-seq datasets were assembled de novo using Trinity and annotated with Trinotate software. After assembly and post-processing steps, 106093 transcripts with N50 of 2371 bp and mean sequence length of 1343.98 bp was produced. BUSCO analyses showed a transcriptome with gene completeness of 97.5 % Arthropoda library profile. The Bowtie2, RSEM and edgeR tools were used for the differential gene expression (DEGs) analyses that allowed the identification of a high quantity of genes differentially expressed in all generations. Finally, to identify the main metabolic pathways affected by the transgenerational effects of SIM across all generations, the DGEs genes were blasted onto KEGG pathways database using the KAAS webserver. The data furnished in this article allows a better molecular understanding of the transgenerational effects produced by simvastatin in the keystone amphipod G. locusta and has major implications for hazard and risk assessment of pharmaceuticals and other emerging contaminants. This article is related to the research article entitled "Transgenerational inheritance of chemical-induced signature: a case study with simvastatin [1].

Keywords: Differential gene expression; Gammarus locusta, Transgenerational effects; RNA-Seq; Simvastatin Metabolic pathways; Transcriptome analysis.