Spectral editing in 1 H magnetic resonance spectroscopy: Experts' consensus recommendations

NMR Biomed. 2021 May;34(5):e4411. doi: 10.1002/nbm.4411. Epub 2020 Sep 18.


Spectral editing in in vivo 1 H-MRS provides an effective means to measure low-concentration metabolite signals that cannot be reliably measured by conventional MRS techniques due to signal overlap, for example, γ-aminobutyric acid, glutathione and D-2-hydroxyglutarate. Spectral editing strategies utilize known J-coupling relationships within the metabolite of interest to discriminate their resonances from overlying signals. This consensus recommendation paper provides a brief overview of commonly used homonuclear editing techniques and considerations for data acquisition, processing and quantification. Also, we have listed the experts' recommendations for minimum requirements to achieve adequate spectral editing and reliable quantification. These include selecting the right editing sequence, dealing with frequency drift, handling unwanted coedited resonances, spectral fitting of edited spectra, setting up multicenter clinical trials and recommending sequence parameters to be reported in publications.

Keywords: J-difference editing; consensus recommendations; multiple quantum filtering; spectral editing; γ-aminobutyric acid, glutathione.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calibration
  • Consensus*
  • Expert Testimony
  • Glioma / genetics
  • Humans
  • Isocitrate Dehydrogenase / genetics
  • Metabolome
  • Motor Cortex / metabolism
  • Mutation / genetics
  • Occipital Lobe / metabolism
  • Proton Magnetic Resonance Spectroscopy*


  • Isocitrate Dehydrogenase