Luteimonas weifangensis sp. nov., Isolated from Bensulfuron-Methyl Contaminated Watermelon Soil

Curr Microbiol. 2020 Nov;77(11):3787-3792. doi: 10.1007/s00284-020-02210-0. Epub 2020 Sep 18.

Abstract

A Gram-stain-negative bacterium, designated WF-2T, was isolated from bensulfuron-methyl contaminated watermelon soil in Weifang, Shandong province, China. Cells of strain WF-2T were strictly aerobic, non-motile and rod-shaped. Strain WF-2T grew optimally at 30 °C, pH 7.0. Strain WF-2T possessed ubiquinone-8 (Q-8) as the predominant respiratory quinone. The major cellular fatty acids of the strain WF-2T (> 5.0%) were iso-C15:0, anteiso-C15:0, iso-C16:0, C16:0, iso-C17:0, and summed feature 9 (iso-C17:1 ω9c/C16:0 10-methyl). The polar lipids consisted of two unidentified lipids, three unidentified phospholipids, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidyl glycolipid. Phylogenetic analysis based on 16S rRNA gTne sequences revealed that WF-2T was a member of the genus Luteimonas and showed the highest sequence similarity to Luteimonas lumbrici 1.1416T (98.6%) and Lysobacter pocheonensis Gsoil 193T (97.9%), lower sequence similarity (< 97.0%) to other known species. The genomic DNA G + C content of WF-2T was 69.2 mol%. Average nucleotide identity (ANI) and the digital DNA-DNA hybridizations (DDH) between strains WF-2T and L. lumbrici 1.1416T were 81.9% and 24.7%, respectively. Based on the phylogenetic, chemotaxonomic and phenotypic data presented here, a new species with the name Luteimonas weifangensis sp. nov. is proposed. The type strain is WF-2T (= KCTC 62441T = CGMCC 1.13633T).

MeSH terms

  • Bacterial Typing Techniques
  • China
  • Citrullus*
  • DNA, Bacterial / genetics
  • Fatty Acids
  • Lysobacter
  • Phospholipids
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology
  • Soil*

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Soil

Supplementary concepts

  • Lysobacter pocheonensis