Synthesis and characterization of magnetic molecularly imprinted polymers for effective extraction and determination of kaempferol from apple samples

Yang Cheng; Jiyun Nie; Hongdi Liu; Lixue Kuang; Guofeng Xu

doi:10.1016/j.chroma.2020.461531

Synthesis and characterization of magnetic molecularly imprinted polymers for effective extraction and determination of kaempferol from apple samples

J Chromatogr A. 2020 Sep 12:1630:461531. doi: 10.1016/j.chroma.2020.461531. Online ahead of print.

Authors

Yang Cheng¹, Jiyun Nie², Hongdi Liu¹, Lixue Kuang¹, Guofeng Xu¹

Affiliations

¹ Institute of Pomology, Chinese Academy of Agricultural Sciences/Laboratory of Quality & Safety Risk Assessment for Fruit (Xingcheng), Ministry of Agriculture and Rural Affairs/Supervision &Test Center of Fruit and Nursery Stocks Quality (Xingcheng), Ministry of Agriculture and Rural Affairs, Xingcheng 125100, PR China.
² College of Horticulture, Qingdao Agriculture University/Qingdao Key Lab of Modern Agriculture Quality and Safety Engineering, Qingdao 266109, PR China; Institute of Pomology, Chinese Academy of Agricultural Sciences/Laboratory of Quality & Safety Risk Assessment for Fruit (Xingcheng), Ministry of Agriculture and Rural Affairs/Supervision &Test Center of Fruit and Nursery Stocks Quality (Xingcheng), Ministry of Agriculture and Rural Affairs, Xingcheng 125100, PR China. Electronic address: niejiyun@caas.cn.

PMID: 32950815
DOI: 10.1016/j.chroma.2020.461531

Abstract

The specific of magnetic molecularly imprinted polymers (Fe₃O₄@SiO₂-MIPs) for kaempferol were fabricated by using acrylamide (AM) as the functional monomer, azobisisobutyronitrile (AIBN) as the initiator and ethylene glycol dimethacrylate (EGDMA) as the cross-linker. The Fe₃O₄@SiO₂-MIPs showed high adsorption capacity (3.84 mg/g) for kaempferol, and the adsorption equilibrium was achieved within 50 min. The specific recognition capacity of Fe₃O₄@SiO₂-MIPs was 3.02 times as high as that of Fe₃O₄@SiO₂-NIPs. The Fe₃O₄@SiO₂-MIPs showed high selectivity towards kaempferol over structural analogues. The recoveries of proposed method at three spiked levels analysis were ranged from 90.5% to 95.4% with the relative standard deviations (RSD) less than 5%. The obtained Fe₃O₄@SiO₂-MIPs were successfully applied for the extraction and determination of kaempferol from apple samples. The established method was simple and feasible, which showed high selectivity, fast separation and satisfactory recoveries for real sample analysis.

Keywords: Apple samples; Kaempferol; Magnetic molecularly imprinted polymers; Selective extraction; Surface molecular imprinting.