A newly established cell line of rabbit lens epithelium

Jpn J Ophthalmol. 1986;30(4):367-75.

Abstract

Rabbit lens epithelial cells have been cultured continuously for more than 24 months (200 generations) in monolayers. Their morphology resembled cobblestones when confluent and was spindle-shaped during growth. The doubling time was 40 hours. The cells were capable of colony formation and their plating efficiency was about 6%. They had immunoreactivity to antiserum to the crystalline-rich supernatant of rabbit lens homogenate. Therefore, it was concluded that they were a permanent cell line and they were named TOTL-86 cells.

MeSH terms

  • Animals
  • Cell Division
  • Cell Line
  • Colony-Forming Units Assay
  • Cytological Techniques*
  • Epithelial Cells
  • Lens, Crystalline / cytology*
  • Rabbits
  • Time Factors