An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis

Nucleic Acids Res. 1987 Jun 11;15(11):4481-9. doi: 10.1093/nar/15.11.4481.

Abstract

The three chromosomal DNAs of S. pombe have been fractionated by pulsed field gel electrophoresis. The resulting molecular karyotype will greatly speed gene mapping in this organism, and it indicates that the separation range of the technique extends to DNA molecules as large as 9,000,000 base pairs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosomes / analysis*
  • DNA, Fungal / genetics*
  • Electrophoresis, Agar Gel / methods
  • Molecular Weight
  • Plasmodium falciparum / genetics
  • Saccharomyces cerevisiae / genetics
  • Saccharomycetales / genetics*
  • Schizosaccharomyces / genetics*

Substances

  • DNA, Fungal