Mycosynthesis of ZnO Nanoparticles Using Trichoderma spp. Isolated from Rhizosphere Soils and Its Synergistic Antibacterial Effect against Xanthomonas oryzae pv. oryzae

Balagangadharaswamy Shobha; Thimappa Ramachandrappa Lakshmeesha; Mohammad Azam Ansari; Ahmad Almatroudi; Mohammad A Alzohairy; Sumanth Basavaraju; Ramesha Alurappa; Siddapura Ramachandrappa Niranjana; Srinivas Chowdappa

doi:10.3390/jof6030181

Mycosynthesis of ZnO Nanoparticles Using Trichoderma spp. Isolated from Rhizosphere Soils and Its Synergistic Antibacterial Effect against Xanthomonas oryzae pv. oryzae

J Fungi (Basel). 2020 Sep 20;6(3):181. doi: 10.3390/jof6030181.

Authors

Balagangadharaswamy Shobha¹, Thimappa Ramachandrappa Lakshmeesha¹, Mohammad Azam Ansari², Ahmad Almatroudi³, Mohammad A Alzohairy³, Sumanth Basavaraju¹, Ramesha Alurappa¹, Siddapura Ramachandrappa Niranjana⁴, Srinivas Chowdappa¹

Affiliations

¹ Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bengaluru 560056, India.
² Department of Epidemic Disease Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia.
³ Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Qassim, 51431 Saudi Arabia.
⁴ Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570006, India.

Abstract

The Plant Growth Promoting Fungi (PGPF) is used as a source of biofertilizers due to their production of secondary metabolites and beneficial effects on plants. The present work is focused on the co-cultivation of Trichoderma spp. (T. harzianum (PGT4), T. reesei (PGT5) and T. reesei (PGT13)) and the production of secondary metabolites from mono and co-culture and mycosynthesis of zinc oxide nanoparticles (ZnO NPs), which were characterized by a UV visible spectrophotometer, Powder X-ray Diffraction (PXRD), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive Spectroscopy (EDAX) and Transmission Electron Microscope (TEM) and Selected Area (Electron) Diffraction (SAED) patterns. The fungal secondary metabolite crude was extracted from the mono and co-culture of Trichoderma spp. And were analyzed by GC-MS, which was further subjected for antibacterial activity against Xanthomonas oryzae pv. Oryzae, the causative organism for Bacterial Leaf Blight (BLB) in rice. Our results showed that the maximum zone of inhibition was recorded from the co-culture of Trichoderma spp. rather than mono cultures, which indicates that co-cultivation of beneficial fungi can stimulate the synthesis of novel secondary metabolites better than in monocultures. ZnO NPs were synthesized from fungal secondary metabolites of mono cultures of Trichoderma harzianum (PGT4), Trichoderma reesei (PGT5), Trichoderma reesei (PGT13) and co-culture (PGT4 + PGT5 + PGT13). These ZnO NPs were checked for antibacterial activity against Xoo, which was found to be of a dose-dependent manner. In summary, the biosynthesized ZnO NPs and secondary metabolites from co-culture of Trichoderma spp. are ecofriendly and can be used as an alternative for chemical fertilizers in agriculture.

Keywords: Trichoderma spp.; ZnO nanoparticles; antibacterial activity Xanthomonas oryzae pv. oryzae; co-cultivation; fungal nanotechnology; secondary metabolites.