Effects of a surface prereacted glass-ionomer filler coating material on biofilm formation and inhibition of dentin demineralization

Clin Oral Investig. 2021 Feb;25(2):683-690. doi: 10.1007/s00784-020-03577-x. Epub 2020 Sep 23.


Objectives: This study investigated the ability of a surface prereacted glass-ionomer (S-PRG) coating material to inhibit the biofilm formation and demineralization of dentin.

Methods and materials: Dentin specimens were randomly divided into three groups: (1) no coating (control), (2) S-PRG filler-containing coat, and (3) a nonS-PRG filler-containing coat. Streptococcus mutans biofilms were grown on the dentin surfaces in a microcosm for 20 h. Then, the quantity of bacteria and water-insoluble glucan in the retained biofilm on the dentin surface were measured. Regarding demineralization inhibition test, specimens were demineralized for 5 days then sectioned into halves and observed under confocal laser scanning microscope (CLSM). One-way ANOVA and Tukey's HSD were used for statistical analysis.

Results: The estimated mean surface roughness for specimens in the S-PRG group was statistically significantly higher than the estimates for both the nonS-PRG and the control group specimens. The quantity of bacteria and water-insoluble glucan/mm2 revealed that the S-PRG group prevented biofilm formation and bacterial adhesion to the dentin surface compared with the control and nonS-PRG groups. The S-PRG group recorded the highest acid-resistance ability with no surface loss.

Conclusion: Application of S-PRG barrier coat on dentin surfaces can inhibit biofilm formation as well as protecting the dentin surface against demineralization.

Clinical significance: Coating material containing S-PRG fillers might be used for caries prevention, through inhibiting biofilm formation, enhancing mineralization, and reducing acidic attack by cariogenic bacteria.

Keywords: Antibacterial; Biofilm; Demineralization; Dentin; Oral biofilm reactor; S-PRG.

MeSH terms

  • Biofilms
  • Dental Caries*
  • Dentin
  • Glass Ionomer Cements / pharmacology
  • Humans
  • Streptococcus mutans
  • Tooth Demineralization* / prevention & control


  • Glass Ionomer Cements