RING finger protein RGLG1 and RGLG2 negatively modulate MAPKKK18 mediated drought stress tolerance in Arabidopsis

doi:10.1111/jipb.13019

. 2021 Mar;63(3):484-493.

doi: 10.1111/jipb.13019.

RING finger protein RGLG1 and RGLG2 negatively modulate MAPKKK18 mediated drought stress tolerance in Arabidopsis

Jiayi Yu¹, Lu Kang¹, Yuanyuan Li¹, Changai Wu¹, Chengchao Zheng¹, Pei Liu², Jinguang Huang¹

Affiliations

¹ State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.
² College of Food Science and Engineering, Shandong Agricultural University, Tai'an, 271018, China.

PMID: 32970364
DOI: 10.1111/jipb.13019

RING finger protein RGLG1 and RGLG2 negatively modulate MAPKKK18 mediated drought stress tolerance in Arabidopsis

Jiayi Yu et al. J Integr Plant Biol. 2021 Mar.

. 2021 Mar;63(3):484-493.

doi: 10.1111/jipb.13019.

Authors

Jiayi Yu¹, Lu Kang¹, Yuanyuan Li¹, Changai Wu¹, Chengchao Zheng¹, Pei Liu², Jinguang Huang¹

Affiliations

¹ State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.
² College of Food Science and Engineering, Shandong Agricultural University, Tai'an, 271018, China.

PMID: 32970364
DOI: 10.1111/jipb.13019

Abstract

Mitogen activated protein kinase kinase kinase 18 (MAPKKK18) mediated signaling cascade plays important roles in Arabidopsis drought stress tolerance. However, the post-translational modulation patterns of MAPKKK18 are not characterized. In this study, we found that the protein level of MAPKKK18 was tightly controlled by the 26S proteasome. Ubiquitin ligases RGLG1 and RGLG2 ubiquitinated MAPKKK18 at lysine residue K32 and K154, and promoted its degradation. Deletion of RGLG1 and RGLG2 stabilized MAPKKK18 and further enhanced the drought stress tolerance of MAPKKK18-overexpression plants. Our data demonstrate that RGLG1 and RGLG2 negatively regulate MAPKKK18-mediated drought stress tolerance in Arabidopsis.

Keywords: Arabidopsis; E3 ligase; MAPKKK18; RGLG1; RGLG2; drought stress; ubiquitination.

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References

REFERENCES

1. Belda-Palazon, B., Julian, J., Coego, A., Wu, Q., Zhang, X., Batistic, O., Alquraishi, S.A., Kudla, J., An, C., and Rodriguez, P.L. (2019). ABA inhibits myristoylation and induces shuttling of the RGLG1 E3 ligase to promote nuclear degradation of PP2CA. Plant J. 98: 813-825.
1. Caron, P., Pankotai, T., Wiegant, W.W., Tollenaere, M.A.X., Furst, A., Bonhomme, C., Helfricht, A., de Groot, A., Pastink, A., Vertegaal, A.C.O., Luijsterburg, M.S., Soutoglou, E., and van Attikum, H. (2019). WWP2 ubiquitylates RNA polymerase II for DNA-PK-dependent transcription arrest and repair at DNA breaks. Genes Dev. 33: 684-704.
1. Chardin, C., Schenk, S.T., Hirt, H., Colcombet, J., and Krapp, A. (2017). Review: Mitogen-Activated Protein Kinases in nutritional signaling in Arabidopsis. Plant Sci. 260: 101-108.
1. Chen, H., Feng, H., Zhang, X., Zhang, C., Wang, T., and Dong, J. (2019). An Arabidopsis E3 ligase HUB2 increases histone H2B monoubiquitination and enhances drought tolerance in transgenic cotton. Plant Biotechnol. J. 17: 556-568.
1. Chen, H., Zou, Y., Shang, Y., Lin, H., Wang, Y., Cai, R., Tang, X., and Zhou, J.M. (2008). Firefly luciferase complementation imaging assay for protein-protein interactions in plants. Plant Physiol. 146: 368-376.

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[1] Belda-Palazon, B., Julian, J., Coego, A., Wu, Q., Zhang, X., Batistic, O., Alquraishi, S.A., Kudla, J., An, C., and Rodriguez, P.L. (2019). ABA inhibits myristoylation and induces shuttling of the RGLG1 E3 ligase to promote nuclear degradation of PP2CA. Plant J. 98: 813-825.

[2] Belda-Palazon, B., Julian, J., Coego, A., Wu, Q., Zhang, X., Batistic, O., Alquraishi, S.A., Kudla, J., An, C., and Rodriguez, P.L. (2019). ABA inhibits myristoylation and induces shuttling of the RGLG1 E3 ligase to promote nuclear degradation of PP2CA. Plant J. 98: 813-825.

[3] Caron, P., Pankotai, T., Wiegant, W.W., Tollenaere, M.A.X., Furst, A., Bonhomme, C., Helfricht, A., de Groot, A., Pastink, A., Vertegaal, A.C.O., Luijsterburg, M.S., Soutoglou, E., and van Attikum, H. (2019). WWP2 ubiquitylates RNA polymerase II for DNA-PK-dependent transcription arrest and repair at DNA breaks. Genes Dev. 33: 684-704.

[4] Caron, P., Pankotai, T., Wiegant, W.W., Tollenaere, M.A.X., Furst, A., Bonhomme, C., Helfricht, A., de Groot, A., Pastink, A., Vertegaal, A.C.O., Luijsterburg, M.S., Soutoglou, E., and van Attikum, H. (2019). WWP2 ubiquitylates RNA polymerase II for DNA-PK-dependent transcription arrest and repair at DNA breaks. Genes Dev. 33: 684-704.

[5] Chardin, C., Schenk, S.T., Hirt, H., Colcombet, J., and Krapp, A. (2017). Review: Mitogen-Activated Protein Kinases in nutritional signaling in Arabidopsis. Plant Sci. 260: 101-108.

[6] Chardin, C., Schenk, S.T., Hirt, H., Colcombet, J., and Krapp, A. (2017). Review: Mitogen-Activated Protein Kinases in nutritional signaling in Arabidopsis. Plant Sci. 260: 101-108.

[7] Chen, H., Feng, H., Zhang, X., Zhang, C., Wang, T., and Dong, J. (2019). An Arabidopsis E3 ligase HUB2 increases histone H2B monoubiquitination and enhances drought tolerance in transgenic cotton. Plant Biotechnol. J. 17: 556-568.

[8] Chen, H., Feng, H., Zhang, X., Zhang, C., Wang, T., and Dong, J. (2019). An Arabidopsis E3 ligase HUB2 increases histone H2B monoubiquitination and enhances drought tolerance in transgenic cotton. Plant Biotechnol. J. 17: 556-568.

[9] Chen, H., Zou, Y., Shang, Y., Lin, H., Wang, Y., Cai, R., Tang, X., and Zhou, J.M. (2008). Firefly luciferase complementation imaging assay for protein-protein interactions in plants. Plant Physiol. 146: 368-376.

[10] Chen, H., Zou, Y., Shang, Y., Lin, H., Wang, Y., Cai, R., Tang, X., and Zhou, J.M. (2008). Firefly luciferase complementation imaging assay for protein-protein interactions in plants. Plant Physiol. 146: 368-376.

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RING finger protein RGLG1 and RGLG2 negatively modulate MAPKKK18 mediated drought stress tolerance in Arabidopsis

Affiliations

RING finger protein RGLG1 and RGLG2 negatively modulate MAPKKK18 mediated drought stress tolerance in Arabidopsis

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