DIGGER: exploring the functional role of alternative splicing in protein interactions

Nucleic Acids Res. 2021 Jan 8;49(D1):D309-D318. doi: 10.1093/nar/gkaa768.


Alternative splicing plays a major role in regulating the functional repertoire of the proteome. However, isoform-specific effects to protein-protein interactions (PPIs) are usually overlooked, making it impossible to judge the functional role of individual exons on a systems biology level. We overcome this barrier by integrating protein-protein interactions, domain-domain interactions and residue-level interactions information to lift exon expression analysis to a network level. Our user-friendly database DIGGER is available at https://exbio.wzw.tum.de/digger and allows users to seamlessly switch between isoform and exon-centric views of the interactome and to extract sub-networks of relevant isoforms, making it an essential resource for studying mechanistic consequences of alternative splicing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Binding Sites
  • Computational Biology / methods
  • Databases, Protein*
  • Exons*
  • Humans
  • Internet
  • Models, Molecular
  • Protein Binding
  • Protein Biosynthesis
  • Protein Conformation, alpha-Helical
  • Protein Conformation, beta-Strand
  • Protein Interaction Domains and Motifs
  • Protein Interaction Mapping / methods*
  • Protein Isoforms
  • Proteome / chemistry*
  • Proteome / genetics
  • Proteome / metabolism
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Software
  • Thermodynamics


  • Protein Isoforms
  • Proteome
  • RNA, Messenger