Evidence for an inhibitory A1 subtype adenosine receptor on pancreatic insulin-secreting cells

Eur J Pharmacol. 1987 Apr 7;136(1):109-12. doi: 10.1016/0014-2999(87)90786-2.


The effects of L- and D-phenylisopropyladenosine (L- and D-PIA) were studied on glucose-induced insulin secretion from the isolated perfused rat pancreas. L-PIA at the low dose of 16.5 nM inhibited insulin secretion by 50%. In contrast, D-PIA at 16.5 and 82.5 nM was ineffective. D-PIA used at a 100-fold higher concentration (1.65 microM) than L-PIA induced a similar inhibition of insulin secretion. The inhibitory effect of L-PIA was abolished by 8-phenyltheophylline (1 microM), a potent P1 purinoceptor antagonist. The present experiments provide evidence for an adenosine receptor of the A1 subtype on the insulin-secreting pancreatic cell of rats.

MeSH terms

  • Animals
  • In Vitro Techniques
  • Insulin / metabolism
  • Insulin Secretion
  • Islets of Langerhans / cytology
  • Islets of Langerhans / metabolism*
  • Phenylisopropyladenosine / pharmacology
  • Rats
  • Rats, Inbred Strains
  • Receptors, Purinergic / metabolism*
  • Stereoisomerism
  • Theophylline / analogs & derivatives
  • Theophylline / pharmacology


  • Insulin
  • Receptors, Purinergic
  • Phenylisopropyladenosine
  • Theophylline
  • 8-phenyltheophylline