Abstract
Mitochondrial NADH dehydrogenase from bovine heart was photolabelled with the inhibitor [3H]dihydrorotenone. A constituent of the hydrophobic domain of the enzyme of Mr 33,000 was the major site of labelling. The identity of this protein with the mitochondrially encoded ND-1 gene product was established by immunoblotting and immunoprecipitation with an antiserum raised to the expected C-terminal sequence of the human ND-1 gene product.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cattle
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Cytochrome Reductases / metabolism*
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Humans
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Immunochemistry
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Immunologic Techniques
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Mitochondria, Heart / enzymology*
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NADH Dehydrogenase / antagonists & inhibitors
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NADH Dehydrogenase / genetics
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NADH Dehydrogenase / metabolism*
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Photochemistry
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Protein Binding
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Rotenone / analogs & derivatives*
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Rotenone / metabolism
Substances
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Rotenone
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1',2'-dihydrorotenone
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Cytochrome Reductases
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NADH Dehydrogenase