Photolabelling of a mitochondrially encoded subunit of NADH dehydrogenase with [3H]dihydrorotenone

FEBS Lett. 1987 Jul 13;219(1):108-12. doi: 10.1016/0014-5793(87)81200-0.

Abstract

Mitochondrial NADH dehydrogenase from bovine heart was photolabelled with the inhibitor [3H]dihydrorotenone. A constituent of the hydrophobic domain of the enzyme of Mr 33,000 was the major site of labelling. The identity of this protein with the mitochondrially encoded ND-1 gene product was established by immunoblotting and immunoprecipitation with an antiserum raised to the expected C-terminal sequence of the human ND-1 gene product.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cytochrome Reductases / metabolism*
  • Humans
  • Immunochemistry
  • Immunologic Techniques
  • Mitochondria, Heart / enzymology*
  • NADH Dehydrogenase / antagonists & inhibitors
  • NADH Dehydrogenase / genetics
  • NADH Dehydrogenase / metabolism*
  • Photochemistry
  • Protein Binding
  • Rotenone / analogs & derivatives*
  • Rotenone / metabolism

Substances

  • Rotenone
  • 1',2'-dihydrorotenone
  • Cytochrome Reductases
  • NADH Dehydrogenase