Immunodiversity of the Arabidopsis ZAR1 NLR Is Conveyed by Receptor-Like Cytoplasmic Kinase Sensors

Alexandre Martel; Bradley Laflamme; Derek Seto; D Patrick Bastedo; Marcus M Dillon; Renan N D Almeida; David S Guttman; Darrell Desveaux

doi:10.3389/fpls.2020.01290

Immunodiversity of the Arabidopsis ZAR1 NLR Is Conveyed by Receptor-Like Cytoplasmic Kinase Sensors

Front Plant Sci. 2020 Aug 21:11:1290. doi: 10.3389/fpls.2020.01290. eCollection 2020.

Authors

Alexandre Martel¹, Bradley Laflamme¹, Derek Seto¹, D Patrick Bastedo¹, Marcus M Dillon¹, Renan N D Almeida¹, David S Guttman^{1

2}, Darrell Desveaux^{1

2}

Affiliations

¹ Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada.
² Centre for the Analysis of Genome Evolution & Function, University of Toronto, Toronto, ON, Canada.

Abstract

The Arabidopsis nucleotide-binding leucine-rich repeat protein ZAR1 can recognize at least six distinct families of pathogenic effector proteins to mount an effector-triggered immune response. This remarkable immunodiversity appears to be conveyed by receptor-like cytoplasmic kinase (RLCK) complexes, which associate with ZAR1 to sense several effector-induced kinase perturbations. Here we show that the recently identified ZAR1-mediated immune responses against the HopX1, HopO1, and HopBA1 effector families of Pseudomonas syringae rely on an expanded diversity of RLCK sensors. We show that individual sensors can recognize distinct effector families, thereby contributing to the expanded surveillance potential of ZAR1 and supporting its role as a guardian of the plant kinome.

Keywords: Arabidopsis; Pseudomonas syringae; ZAR1; ZED/ZRK; effector-triggered immunity; immunodiversity; receptor-like cytoplasmic kinase (RLCK).