[Proton translocation in membranes of submitochondrial particles]

Biokhimiia. 1977 Jun;42(6):1123-7.
[Article in Russian]

Abstract

Effect of an electrophilous inhibitor, chlorophenacyl, on energy-dependent functions of submitochondrial particles is studied. Chlorophenacyl at concentrations up to 1 mM is found practically not to affect the generation of membrane potential under NADH and succinate oxidation and ATP hydrolysis and to be a strong inhibitor of oxidative phosphorylation and reverse electron transport. The mechanism of the inhibition of energy-dependent functions of submitochondrial particles with chlorophenacyl is different from that of electron transport inhibitor, energy transport inhibitors and classical uncoupling agents--protonophors. The data obtained are suggested to be due to the existence of two ways of proton translocation in submitochondrial particle membrane, phosphorylating and non-phosphorylating, the effect of chlorophenacyl being directed on phosphorylating way only.

Publication types

  • English Abstract

MeSH terms

  • Biological Transport / drug effects
  • Dose-Response Relationship, Drug
  • Electron Transport / drug effects
  • Energy Transfer / drug effects
  • Membrane Potentials / drug effects
  • Membranes / physiology
  • Mitochondria, Muscle / metabolism*
  • Myocardium / metabolism
  • Oxidative Phosphorylation / drug effects
  • Protons*
  • Uncoupling Agents / pharmacology
  • omega-Chloroacetophenone / pharmacology*

Substances

  • Protons
  • Uncoupling Agents
  • omega-Chloroacetophenone