Generation and characterization of two induced pluripotent stem cell lines (NUIGi038-A, NUIGi038-B) from dermal fibroblasts of a healthy individual

Ning Ge; Min Liu; Xiaoran Zhu; Janusz Krawczyk; Veronica McInerney; Sanbing Shen; Timothy O'Brien; Terence Prendiville

doi:10.1016/j.scr.2020.101996

Generation and characterization of two induced pluripotent stem cell lines (NUIGi038-A, NUIGi038-B) from dermal fibroblasts of a healthy individual

Stem Cell Res. 2020 Dec:49:101996. doi: 10.1016/j.scr.2020.101996. Epub 2020 Sep 16.

Authors

Ning Ge¹, Min Liu², Xiaoran Zhu³, Janusz Krawczyk⁴, Veronica McInerney⁵, Sanbing Shen⁶, Timothy O'Brien⁷, Terence Prendiville⁸

Affiliations

¹ Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI) Galway, Ireland.
² Department of Physiology, College of Life Science, Hebei Normal University, Shijiazhuang, China.
³ Department of Pharmacy, Hebei General Hospital, Shijiazhuang, China.
⁴ Department of Haematology, Galway University Hospital, Ireland.
⁵ HRB Clinical Research Facility, National University of Ireland (NUI) Galway, Ireland.
⁶ Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI) Galway, Ireland. Electronic address: Sanbing.Shen@nuigalway.ie.
⁷ Regenerative Medicine Institute, School of Medicine, National University of Ireland (NUI) Galway, Ireland. Electronic address: Timothy.OBrien@nuigalway.ie.
⁸ National Children's Research Centre, Children's Health Ireland at Crumlin, Dublin 12, Ireland. Electronic address: Terence.Prendiville@olchc.ie.

PMID: 33002719
DOI: 10.1016/j.scr.2020.101996

Abstract

Two human induced pluripotent stem cell (hiPSC) lines (NUIGi038-A, NUIGi038-B) were generated from dermal fibroblasts of a healthy 47 year old female using non-integrational Sendai reprogramming method expressing OCT4, SOX2, KLF4 and C-MYC. Characterization of both hiPSC lines was confirmed by the expression of typical pluripotency markers and differentiation potential in vitro.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cellular Reprogramming
Female
Fibroblasts
Humans
Induced Pluripotent Stem Cells*
Kruppel-Like Factor 4
Middle Aged