Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis

Sci Rep. 2020 Oct 1;10(1):16301. doi: 10.1038/s41598-020-73199-6.


Enterococcus faecalis is a gram-positive organism responsible for serious infections in humans, but as with many bacterial pathogens, resistance has rendered a number of commonly used antibiotics ineffective. Here, we report the cryo-EM structure of the E. faecalis 70S ribosome to a global resolution of 2.8 Å. Structural differences are clustered in peripheral and solvent exposed regions when compared with Escherichia coli, whereas functional centres, including antibiotic binding sites, are similar to other bacterial ribosomes. Comparison of intersubunit conformations among five classes obtained after three-dimensional classification identifies several rotated states. Large ribosomal subunit protein bL31, which forms intersubunit bridges to the small ribosomal subunit, assumes different conformations in the five classes, revealing how contacts to the small subunit are maintained throughout intersubunit rotation. A tRNA observed in one of the five classes is positioned in a chimeric pe/E position in a rotated ribosomal state. The 70S ribosome structure of E. faecalis now extends our knowledge of bacterial ribosome structures and may serve as a basis for the development of novel antibiotic compounds effective against this pathogen.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Anti-Bacterial Agents / metabolism
  • Binding Sites
  • Cryoelectron Microscopy
  • Enterococcus faecalis / ultrastructure*
  • Escherichia coli / metabolism
  • Escherichia coli / ultrastructure
  • Protein Conformation
  • Ribosome Subunits, Large / metabolism
  • Ribosome Subunits, Large / ultrastructure*


  • Anti-Bacterial Agents