Characterization of protein kinase C from normal and transformed cultured murine fibroblasts

Biochem Biophys Res Commun. 1987 Jul 15;146(1):140-6. doi: 10.1016/0006-291x(87)90702-9.


Protein kinase C of normal and ras-transformed NIH 3T3 cells was purified by chromatography on TSK DEAE-5PW, threonine-Sepharose, and TSK phenyl-5PW columns. Comparison of the fibroblast enzyme with several types of rat brain protein kinase C by chromatography on a hydroxyapatite column and by immunoblotting, indicates that both normal and transformed fibroblasts possess only one of the four subspecies of protein kinase C which have been identified in brain tissues. This subspecies presumably has the structure encoded by alpha-sequence or a closely related sequence. No significant difference was seen between those enzymes purified from normal and transformed fibroblasts.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Brain / enzymology
  • Cell Transformation, Neoplastic*
  • Cells, Cultured
  • Chromatography, Gel
  • Fibroblasts / enzymology*
  • Immunosorbent Techniques
  • Mice
  • Oncogenes
  • Protein Kinase C / metabolism*
  • Rats


  • Antibodies, Monoclonal
  • Protein Kinase C