Characterization and comparison of collagen extracted from the skin of the Nile tilapia by fermentation and chemical pretreatment

Zengliu Song; Huanming Liu; Liwen Chen; Leilei Chen; Chunxia Zhou; Pengzhi Hong; Chujin Deng

doi:10.1016/j.foodchem.2020.128139

Characterization and comparison of collagen extracted from the skin of the Nile tilapia by fermentation and chemical pretreatment

Food Chem. 2021 Mar 15:340:128139. doi: 10.1016/j.foodchem.2020.128139. Epub 2020 Sep 25.

Authors

Zengliu Song¹, Huanming Liu², Liwen Chen¹, Leilei Chen¹, Chunxia Zhou¹, Pengzhi Hong¹, Chujin Deng¹

Affiliations

¹ Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Marine Food, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
² Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Marine Food, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. Electronic address: liu241253@gdou.edu.cn.

PMID: 33010648
DOI: 10.1016/j.foodchem.2020.128139

Abstract

Chemical pretreatment of collagen raw materials is time-consuming and environmentally hazardous. Collagen extraction after fermentation pretreatment has not been reported. We extracted and characterized acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from Nile tilapia (Oreochromis niloticus) skin following fermentation and chemical treatments and comparatively evaluated the feasibility of fermentation. Fermentation-ASC (FASC) and fermentation-PSC (FPSC) yields (4.76 and 8.14 wt%, respectively) were slightly but not significantly higher than chemical-ASC (CASC) and chemical-PSC (CPSC) yields (4.27 and 7.60 wt%, respectively). All extracts were identified as type I collagens by SDS-PAGE and retained their triple helical structure well, as confirmed through Fourier transform infrared spectroscopy. All collagen microstructures under scanning electron microscopy were multi-layered aggregates. These collagens also had similar biochemical properties (i.e. denatured between 36.5 and 37.1 °C, high soluble at pH 1-4 and at <3% [w/v] NaCl). Therefore, fermentation method is a viable alternative for pretreating collagen extraction materials.

Keywords: Collagen; Fermentation; Fish skin; Oreochromis niloticus; Pretreatment.

MeSH terms

Amino Acids / analysis
Animals
Bacillus / growth & development
Cichlids / metabolism*
Collagen Type I / chemistry*
Collagen Type I / metabolism
Fish Proteins / chemistry*
Fish Proteins / metabolism
Hydrogen-Ion Concentration
Microscopy, Electron, Scanning
Pepsin A / metabolism
Skin / metabolism
Sodium Chloride / chemistry
Solubility
Spectroscopy, Fourier Transform Infrared

Substances

Amino Acids
Collagen Type I
Fish Proteins
Sodium Chloride
Pepsin A

Supplementary concepts

Bacillus velezensis