Generation of induced pluripotent stem cells (NIMHi002-A and NIMHi003-A) from two sporadic Parkinson's disease patient of East Indian ethnicity

Indrani Datta; Soham Jagtap; Sowmithra; Ravi Yadav; Pramod Kumar Pal

doi:10.1016/j.scr.2020.101995

Generation of induced pluripotent stem cells (NIMHi002-A and NIMHi003-A) from two sporadic Parkinson's disease patient of East Indian ethnicity

Stem Cell Res. 2020 Dec:49:101995. doi: 10.1016/j.scr.2020.101995. Epub 2020 Sep 17.

Authors

Indrani Datta¹, Soham Jagtap², Sowmithra², Ravi Yadav³, Pramod Kumar Pal³

Affiliations

¹ Department of Biophysics, National Institute of Mental Health and Neurosciences, Bengaluru 560029, India. Electronic address: indranidatta.nimhans@gmail.com.
² Department of Biophysics, National Institute of Mental Health and Neurosciences, Bengaluru 560029, India.
³ Department of Neurology, National Institute of Mental Health and Neurosciences, Bengaluru 560029, Karnataka, India.

PMID: 33010678
DOI: 10.1016/j.scr.2020.101995

Abstract

Epidemiological studies suggest that about 95% of PD have a sporadic component. We have generated induced pluripotent stem cells (iPSCs) using Sendai-virus reprogramming-method from peripheral blood mononuclear cells of two sporadic PD-patient of East-Indian ethnicity carrying no PD-related gene mutations. PD diagnosis was performed using Unified Parkinson's Disease rating scale (UPDRS) score and confirmed by [¹⁸F]fluoro-L-dopa [F-DOPA] positron emission tomography (F-DOPA PET). The iPSC lines were characterized for self-renewal and pluripotency. These generated lines will provide a valuable resource to understand the pathophysiology of this disease and a drug-screening platform.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ethnicity
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Parkinson Disease*
Sendai virus