Derivation of three human induced pluripotent stem cell lines (JUCTCi014-A, JUCTCi015-A, JUCTCi016-A) from mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue and Wharton's jelly samples

Nidaa A Ababneh; Ban Al-Kurdi; Raghda Barham; Dema Ali; Nour Sharar; Duaa Abuarqoub; Hanan Jafar; Bareqa Salah; Abdalla Awidi

doi:10.1016/j.scr.2020.102000

Derivation of three human induced pluripotent stem cell lines (JUCTCi014-A, JUCTCi015-A, JUCTCi016-A) from mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue and Wharton's jelly samples

Stem Cell Res. 2020 Dec:49:102000. doi: 10.1016/j.scr.2020.102000. Epub 2020 Sep 28.

Authors

Nidaa A Ababneh¹, Ban Al-Kurdi², Raghda Barham², Dema Ali², Nour Sharar², Duaa Abuarqoub³, Hanan Jafar⁴, Bareqa Salah⁵, Abdalla Awidi⁶

Affiliations

¹ Cell Therapy Center, The University of Jordan, Amman, Jordan. Electronic address: n.ababneh@ju.edu.jo.
² Cell Therapy Center, The University of Jordan, Amman, Jordan.
³ Cell Therapy Center, The University of Jordan, Amman, Jordan; Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, Jordan.
⁴ Cell Therapy Center, The University of Jordan, Amman, Jordan; Department of Anatomy and Histology, School of Medicine, The University of Jordan, Amman, Jordan.
⁵ General Surgery Department/Plastic & Reconstructive, Jordan University Hospital, The University of Jordan, Amman, Jordan.
⁶ Cell Therapy Center, The University of Jordan, Amman, Jordan; Hemostasis and Thrombosis Laboratory, School of Medicine, the University of Jordan, Amman, Jordan; Department of Hematology and Oncology, Jordan University Hospital, Amman, Jordan.

PMID: 33010679
DOI: 10.1016/j.scr.2020.102000

Abstract

Mesenchymal stem cells (MSCs) are recognized as a valuable source of cells in clinical treatment and tissue engineering applications. In this study, we created human induced pluripotent stem cells (hiPSCs) from different MSC sources to evaluate the capacity of MSC-derived iPSCs to differentiate into any cell type of the human body and to serve as an alternative source for iPSC generation. Here in, the generated hiPSC lines retained their normal karyotype and showed similar STR-based identities to the parental cells. Reprogrammed cells also showed positive expression of the pluripotency markers and the ability to differentiate into the three germ layers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipose Tissue
Bone Marrow
Bone Marrow Cells
Cell Differentiation
Cells, Cultured
Humans
Induced Pluripotent Stem Cells*
Mesenchymal Stem Cells*
Wharton Jelly*