Anti-inflammatory and antioxidant properties of chemical constituents of Broussonetia papyrifera

Bioorg Chem. 2020 Nov:104:104298. doi: 10.1016/j.bioorg.2020.104298. Epub 2020 Sep 19.

Abstract

Extensive phytochemical analysis of the CHCl3-soluble part of an ethanolic extract of branches and twigs of Broussonetia papyrifera led to the isolation of fourteen compounds, including a novel 5,11-dioxabenzo[b]fluoren-10-one derivative named broussofluorenone C (12). The isolated compounds 1-14 were characterized based on their NMR and HRMS data, and examined for their anti-inflammatory activities in LPS-stimulated THP-1 cells as well as for their cellular antioxidant effects. Compounds 7-10 and 12 showed inhibitory effects on NF-κB/AP-1 activation and compounds 7-9 were subsequently confirmed to suppress the secretion of both IL-1β and TNF-α in LPS-stimulated THP-1 cells more significantly than the prednisone used as a positive control. In the CAA assay, compound 10 exhibited the greatest antioxidant effect, greater than that of the quercetin used as a positive control. The results show possible beneficial effects and utilization of B. papyrifera wood in the treatment of inflammatory diseases as well as oxidative stress.

Keywords: Anti-inflammatory; Antioxidant; Broussonetia papyrifera; NF-κB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology*
  • Antioxidants / chemistry
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology*
  • Broussonetia / chemistry*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Humans
  • Interleukin-1beta / antagonists & inhibitors
  • Interleukin-1beta / biosynthesis
  • Lipopolysaccharides / antagonists & inhibitors
  • Lipopolysaccharides / pharmacology
  • Molecular Structure
  • NF-kappa B / analysis
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / biosynthesis
  • Oxidative Stress / drug effects
  • Structure-Activity Relationship
  • THP-1 Cells
  • Transcription Factor AP-1 / analysis
  • Transcription Factor AP-1 / antagonists & inhibitors
  • Transcription Factor AP-1 / biosynthesis
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors
  • Tumor Necrosis Factor-alpha / biosynthesis

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • IL1B protein, human
  • Interleukin-1beta
  • Lipopolysaccharides
  • NF-kappa B
  • Transcription Factor AP-1
  • Tumor Necrosis Factor-alpha