Abstract
Haemolytic uremic syndrome often affects children causing a relevant morbidity and mortality. We compared the time to diagnosis by multiplex-PCR and stool culture in 15 children from two centres. Multiplex-PCR accelerated the time to diagnosis by 94 (95% confidence interval, 80-119; P = 0.0007) hours. Multiplex-PCR offers a time advantage of stool culture that may aid in earlier identification of outbreak clusters.
Keywords:
Biofire; Children; Gastrointestinal panel; Shiga-like toxin.
Publication types
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Comparative Study
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Multicenter Study
MeSH terms
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Child
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Child, Preschool
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Early Diagnosis
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Escherichia coli Infections / diagnosis
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Escherichia coli Infections / microbiology
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Feces / microbiology*
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Hemolytic-Uremic Syndrome / diagnosis*
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Hemolytic-Uremic Syndrome / microbiology
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Humans
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Infant
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Multiplex Polymerase Chain Reaction*
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Point-of-Care Testing*
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Retrospective Studies
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Shiga-Toxigenic Escherichia coli / genetics
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Shiga-Toxigenic Escherichia coli / isolation & purification