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. 2020 Oct;84(4):310-313.

Mycoplasma hyopneumoniae genetic variability within swine production flows

Alyssa M Betlach  1 , Eduardo Fano  1 , Amanda Sponheim  1 , Robert Valeris-Chacin  1 , Laura Dalquist  1 , Randall S Singer  1 , Maria Pieters  1
Affiliations

Mycoplasma hyopneumoniae genetic variability within swine production flows

Alyssa M Betlach et al. Can J Vet Res. 2020 Oct.

Abstract
in English, French

The aim of this study was to assess the genetic variability of Mycoplasma hyopneumoniae within various swine production flows. Four M. hyopneumoniae positive production flows, composed of 4 production stages, were selected for this study. Laryngeal and/or bronchial swabs were collected from each production stage within a flow, for a period of 4 months up to 3 years. A multiple-locus variable-number tandem repeat analysis was performed to assess the genetic variation of M. hyopneumoniae within and across production flows through the identification of variable-number tandem repeat (VNTR) types. A maximum of 6 M. hyopneumoniae VNTR types were identified in a single flow, in which VNTR types appeared to be flow specific. An identical VNTR type was detected across several production stages for up to 3 years. In this study, minimal M. hyopneumoniae genetic variation was evidenced within and across production flows.

L’objectif de cette étude était d’évaluer la variabilité génétique de Mycoplasma hyopneumoniae au sein de différents flux de production porcine. Quatre flux de production positifs pour M. hyopneumoniae, composés de quatre stades de production, furent sélectionnés pour cette étude. Des écouvillons laryngés et/ou bronchiaux furent prélevés de chaque stade de production à l’intérieur d’un flux, pour une période de 4 mois jusqu’à 3 ans. Une analyse multi-locus du polymorphisme des séquences répétées en tandem fut effectuée afin d’évaluer la variation génétique de M. hyopneumoniae au sein et à travers les flux de production par l’identification des types de polymorphismes de séquences répétées en tandem (VNTR). Un maximum de six types de VNTR de M. hyopneumoniae fut identifié dans un flux unique, dans lequel les types de VNTR apparaissaient être spécifiques de flux. Un type de VNTR identique fut détecté à travers plusieurs stades de production et jusqu’à 3 ans. Dans cette étude, une variation génétique minime de M. hyopneumoniae fut notée au sein et à travers des flux de production.(Traduit par Docteur Serge Messier).

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Conflict of interest statement

Conflict of Interest The author(s) declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. Drs. Fano and Sponheim are employed by Boehringer Ingelheim Animal Health USA Inc. However, no commercial products were evaluated in this investigation. Dr. Singer has received funding from Boehringer Ingelheim Animal Health USA Inc.

Figures

Figure 1
Figure 1
Minimum spanning tree of Mycoplasma hyopneumoniae variable-number tandem repeat (VNTR) types. Circle color corresponds to production flow (i.e., dark gray = Flow A; light gray = Flow B; white = Flow C; black = Flow D). Grey shading illustrates clonal complex of VNTR types. Lines connecting 2 circles show a relationship between the VNTR types, whereas darker lines represent a clonal complex. The 2 numbers outside of each circle correspond to the VNTR type in the sample. Each number represents the number of tandem repeats for P97 and P146 loci, respectively. Circle size corresponds to sample size (the larger the circle, the more samples with the specific VNTR type). For example, n = 1, 2, 4, and 15 for MLVA types 11–23, 11–22, 13–17, and 16–24, respectively.
Figure 2
Figure 2
Mycoplasma hyopneumoniae variable-number tandem repeat (VNTR) types identified over time by flow and production stage. Each box corresponds to a production flow (A to D). Each circle represents a single VNTR type detected in a given herd on a given date. Circle color corresponds to production stage (i.e., black = gilt developing unit, dark gray = sow farm; light gray = nursery; white = finisher). Within each circle, the first and second number correspond to the number of tandem repeats identified in P97 and P146 loci, respectively.
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References

    1. Mare CJ, Switzer WP. New species: Mycoplasma hyopneumoniae; A causative agent of virus pig pneumonia. Vet Med Small Anim Clin. 1965;60:841–846. - PubMed
    1. Goodwin RF, Pomeroy AP, Whittlestone P. Production of enzootic pneumonia in pigs with mycoplasma. Vet Rec. 1965;77:1247–1249.
    1. Pieters MG, Maes D. Mycoplasmosis. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW, Zhang J, editors. Diseases of Swine. 11th ed. Hoboken, New Jersey: John Wiley & Sons; 2019. pp. 863–883.
    1. Vicca J, Maes D, Thermote L, Peeters J, Haesebrouck F, de Kruif A. Patterns of Mycoplasma hyopneumoniae infections in Belgian farrow-to-finish pig herds with diverging disease-course. J Vet Med B Infect Dis Vet Public Health. 2002;49:349–353. - PubMed
    1. Vicca J, Stakenborg T, Maes D, et al. Evaluation of virulence of Mycoplasma hyopneumoniae field isolates. Vet Microbiol. 2003;97:177–190. - PubMed

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