Effects of Exogenous Putrescine on Delaying Senescence of Cut Foliage of Nephrolepis cordifolia

Ying Qu; Lu Jiang; Tana Wuyun; Shouyuan Mu; Fuchun Xie; Yajun Chen; Lu Zhang

doi:10.3389/fpls.2020.566824

Effects of Exogenous Putrescine on Delaying Senescence of Cut Foliage of Nephrolepis cordifolia

Front Plant Sci. 2020 Sep 10:11:566824. doi: 10.3389/fpls.2020.566824. eCollection 2020.

Authors

Ying Qu¹, Lu Jiang¹, Tana Wuyun², Shouyuan Mu¹, Fuchun Xie¹, Yajun Chen¹, Lu Zhang¹

Affiliations

¹ College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China.
² Chair of Crop Science and Plant Biology, Estonian University of Life Sciences, Tartu, Estonia.

Abstract

Senescence is the main limitation for cut foliage display in vase. Naturally occurring polyamines such as putrescine (Put) have been considered effective anti-senescence agents. However, effect of Put on cut foliage in vase in a realistic indoor environment has not yet been revealed. In the present study, effects of Put spraying on the postharvest performance of cut foliage of Nephrolepis cordifolia L. were investigated. Cut fronds sprayed with deionized water (Put0) showed visible injuries after 10 days in vase. Meanwhile, chlorophyll (Chl), soluble protein (Sp), and proline (Pro) content were decreased by 60.15, 57.93, and 73.09% respectively, photochemical activity reflected by Chl fluorescence parameters was inhibited, whereas electrolyte leakage (EL), contents of soluble sugar (Ss), malondialdehyde (MDA), and hydrogen peroxide (H₂O₂) were increased (+194.29, +44.83, +34.06, and +178.01%, respectively). Put spraying extended the vase life of the cut foliage and the 2.0 mM Put had a longer vase life (21 days) than 0.2 mM (15 days). Leaf spraying of 2.0 mM Put for 10 days significantly ameliorated the losses of Chl, Sp, and Pro content (-10.72, -26.29, and -42.64%, respectively), followed by 0.2 mM Put (-27.36, -36.24, and -60.55%, respectively). Put spraying also improved photochemical capability and prevented membrane impairment as well as visible injury in comparison with Put0. In addition, 2.0 mM Put had a better mitigating ability than that of 0.2 mM. Leaf spraying of 2.0 mM Put greatly reduced the decline of the effective quantum yield of photochemical energy conversion in PSII (ΦPSII), the maximal quantum yield of PSII photochemistry measured in the dark-adapted state (Fv/Fm) and electron transport rate (ETR) (-7.89, -12.91, and -10.06%, respectively), and also inhibited the increases of EL, MDA, Ss, and H₂O₂ (+31.87, +6.43, +16.22, and +49.40%, respectively). Overall, Put played important roles in deterring the degradation of Chl, Ss, and Pro, detoxifying the H₂O₂, weakening the sugar signaling, mitigating the decline of photochemical activity, and eventually postponing the leaf senescence. The present study gives new insights into effects of Put on leaf senescence and provides a strategy for preserving post-harvest cut foliage.

Keywords: antioxidant enzyme; biochemical; chlorophyll fluorescence imaging; polyamines; sword fern.