Homocysteine aggravates DNA damage by impairing the FA/Brca1 Pathway in NE4C murine neural stem cells

Int J Med Sci. 2020 Sep 9;17(16):2477-2486. doi: 10.7150/ijms.49246. eCollection 2020.

Abstract

There is existing evidence that elevated homocysteine (Hcy) levels are risk factors for some neurodegenerative disorders. The pathogenesis of neurological diseases could be contributed to excessive cell dysfunction and death caused by defective DNA damage response (DDR) and accumulated DNA damage. Hcy is a neurotoxic amino acid and acts as a DNA damage inducer. However, it is not clear whether Hcy participates in the DDR. To investigate the effects of Hcy on DNA damage and the DDR, we employed mitomycin C (MMC) to cause DNA damage in NE4C murine neural stem cells (NSCs). Compared to treatment with MMC alone, we found that co-treatment with MMC and Hcy worsened DNA damage and increased death in NE4C cells. Intriguingly, in this DNA damage model mimicked by MMC, immunoblotting results showed that the monoubiquitination levels of Fanconi anemia complementation group I (Fanci) and Fanconi anemia complementation group D2 (Fancd2) were decreased to about 60.3% and 55.7% by supplementing cell culture medium with Hcy, indicating Hcy inactivates the function of Fanci and Fancd2 in DNA damage conditions. Given Breast Cancer 1 (BRCA1) is an important downstream of FANCD2, we next detected the interaction between Fancd2 and Brca1 in NE4C cells. Compared to treatment with MMC alone, the Fancd2-Brca1 interaction and the amount of Brca1 on chromatin were decreased when cells were co-exposed to MMC and Hcy, suggesting Hcy could impair the Fanconi anemia (FA)/Brca1 pathway. Taken together, our study demonstrates that Hcy may enhance cell death, which contributes to the accumulation of DNA damage and promotion of hypersensitivity to cytotoxicity by impairing the FA/Brca1 pathway in murine NSCs in the presence of DNA damage.

Keywords: Brca1; DNA damage; Fanconi anemia pathway; homocysteine; neural stem cell.

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Apoptosis / genetics
  • BRCA1 Protein / metabolism
  • Cell Line
  • Chromatin / metabolism
  • Culture Media / metabolism
  • DNA Damage / drug effects
  • DNA Damage / physiology*
  • DNA Repair / drug effects
  • DNA Repair / physiology*
  • Fanconi Anemia Complementation Group D2 Protein / metabolism
  • Fanconi Anemia Complementation Group Proteins / metabolism
  • Homocysteine / blood
  • Homocysteine / metabolism*
  • Humans
  • Mice
  • Mitomycin / toxicity
  • Neural Stem Cells / pathology
  • Neurodegenerative Diseases / blood
  • Neurodegenerative Diseases / genetics*
  • Neurodegenerative Diseases / pathology
  • Signal Transduction / drug effects
  • Signal Transduction / genetics*
  • Ubiquitination / genetics

Substances

  • BRCA1 Protein
  • Brca1 protein, mouse
  • Chromatin
  • Culture Media
  • FANCI protein, mouse
  • Fancd2 protein, mouse
  • Fanconi Anemia Complementation Group D2 Protein
  • Fanconi Anemia Complementation Group Proteins
  • Homocysteine
  • Mitomycin