Regeneration of lingual musculature in rats using myoblasts over porcine bladder acellular matrix

doi:10.1111/odi.13674

. 2021 Sep;27(6):1580-1589.

doi: 10.1111/odi.13674. Epub 2020 Oct 22.

Regeneration of lingual musculature in rats using myoblasts over porcine bladder acellular matrix

Fabio Camacho-Alonso¹, Nuria García-Carrillo², Antonio Julián Buendía³, Jose Antonio Navarro³, David Peñarrocha-Oltra⁴, Miguel Peñarrocha-Diago⁴, Jose Lacal-Luján¹

Affiliations

¹ Department of Oral Surgery, University of Murcia, Murcia, Spain.
² Biomedical Research Center (CEIB), University of Murcia, Murcia, Spain.
³ Department of Histology and Pathology Anatomy, University of Murcia, Murcia, Spain.
⁴ Department of Oral Surgery, University of Valencia, Valencia, Spain.

PMID: 33031620
DOI: 10.1111/odi.13674

Regeneration of lingual musculature in rats using myoblasts over porcine bladder acellular matrix

Fabio Camacho-Alonso et al. Oral Dis. 2021 Sep.

. 2021 Sep;27(6):1580-1589.

doi: 10.1111/odi.13674. Epub 2020 Oct 22.

Authors

Fabio Camacho-Alonso¹, Nuria García-Carrillo², Antonio Julián Buendía³, Jose Antonio Navarro³, David Peñarrocha-Oltra⁴, Miguel Peñarrocha-Diago⁴, Jose Lacal-Luján¹

Affiliations

¹ Department of Oral Surgery, University of Murcia, Murcia, Spain.
² Biomedical Research Center (CEIB), University of Murcia, Murcia, Spain.
³ Department of Histology and Pathology Anatomy, University of Murcia, Murcia, Spain.
⁴ Department of Oral Surgery, University of Valencia, Valencia, Spain.

PMID: 33031620
DOI: 10.1111/odi.13674

Abstract

Objectives: To use tissue engineering muscle repair (TEMR) for regenerating the lingual musculature of hemiglossectomized rats using neonatal myoblasts (NM) on porcine acellular urinary bladder matrix (AUBM).

Material and methods: The study used 80 male rats. A volumetric muscle loss (VML) injury was created on the left side of the tongue. The rats were randomized into four groups: Group 1 (AUBM + myoblasts); Group 2 (AUBM); Group 3 (myoblasts); and Group 4 (control). NM were obtained from neonatal rats. The animals were weighed on day 0 and just before euthanasia. Five rats in each group were euthanized at days 2, 14, 28, and 42; the tongues were prepared for morphometric analysis, postoperative left hemitongue weight, and immunohistochemical analysis (desmin, CD-31, and anti-neurofilament).

Results: The weight gain from greatest to least was as follows: AUBM + myoblasts > myoblasts > AUBM > control. The tongue dorsum occupied by VML, and difference in mg between control side and intervened side from least to great was as follows: AUBM + myoblasts < myoblasts < AUBM < control. The order from highest to lowest antibody positivity was as follows: AUBM + myoblasts > myoblasts > AUBM > control.

Conclusion: The use of porcine AUBM and NM for the regeneration of lingual musculature was found to be an effective TEMR treatment for repairing tongue VML injury.

Keywords: hemiglossectomized tongues; neonatal myoblasts; porcine acellular urinary bladder matrix; tissue engineering muscle repair; volumetric muscle loss.

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Cited by

Distinct Embryonic Origin and Injury Response of Resident Stem Cells in Craniofacial Muscles.
Cheng X, Shi B, Li J. Cheng X, et al. Front Physiol. 2021 Jul 1;12:690248. doi: 10.3389/fphys.2021.690248. eCollection 2021. Front Physiol. 2021. PMID: 34276411 Free PMC article. Review.

References

REFERENCES

1. Aurora, A., Corona, B. T., & Walters, T. J. (2016). A porcine urinary bladder matrix does not recapitulate the spatiotemporal macrophage response of muscle regeneration after volumetric muscle loss injury. Cells Tissues Organs, 202(3-4), 189-201. https://doi.org/10.1159/000447582
1. Aurora, A., Roe, J. L., Corona, B. T., & Walters, T. J. (2015). An acellular biologic scaffold does note regenerate apreciable de novo muscle tissue in rat models of volumetric muscle loss injury. Biomaterials, 67, 393-407. https://doi.org/10.1016/j.biomaterials.2015.07.040
1. Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 68(6), 394-424. https://doi.org/10.3322/caac.21492
1. Camacho-Alonso, F., López-Jornet, P., & Bermejo-Fenoll, A. (2005). Effects of scalpel (with and without tissue adhesive) and cryosurgery on wound healing in rat tongues. Oral Surgery Oral Medicine Oral Pathology Oral Radiology, and Endodontics, 100(3), e58-e63. https://doi.org/10.1016/j.tripleo.2005.04.016
1. Camacho-Alonso, F., Torralba-Ruiz, M. R., García-Carrillo, N., Lacal-Luján, J., Martínez-Díaz, F., & Sánchez-Siles, M. (2019). Effects of topical apllications of porcine acellular urinary bladder matrix and Centella asiatica extract on oral wound healing in a rat model. Clinical Oral Investigations, 23(5), 2083-2095. https://doi.org/10.1007/s00784-018-2620-x

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[1] Aurora, A., Corona, B. T., & Walters, T. J. (2016). A porcine urinary bladder matrix does not recapitulate the spatiotemporal macrophage response of muscle regeneration after volumetric muscle loss injury. Cells Tissues Organs, 202(3-4), 189-201. https://doi.org/10.1159/000447582

[2] Aurora, A., Corona, B. T., & Walters, T. J. (2016). A porcine urinary bladder matrix does not recapitulate the spatiotemporal macrophage response of muscle regeneration after volumetric muscle loss injury. Cells Tissues Organs, 202(3-4), 189-201. https://doi.org/10.1159/000447582

[3] Aurora, A., Roe, J. L., Corona, B. T., & Walters, T. J. (2015). An acellular biologic scaffold does note regenerate apreciable de novo muscle tissue in rat models of volumetric muscle loss injury. Biomaterials, 67, 393-407. https://doi.org/10.1016/j.biomaterials.2015.07.040

[4] Aurora, A., Roe, J. L., Corona, B. T., & Walters, T. J. (2015). An acellular biologic scaffold does note regenerate apreciable de novo muscle tissue in rat models of volumetric muscle loss injury. Biomaterials, 67, 393-407. https://doi.org/10.1016/j.biomaterials.2015.07.040

[5] Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 68(6), 394-424. https://doi.org/10.3322/caac.21492

[6] Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 68(6), 394-424. https://doi.org/10.3322/caac.21492

[7] Camacho-Alonso, F., López-Jornet, P., & Bermejo-Fenoll, A. (2005). Effects of scalpel (with and without tissue adhesive) and cryosurgery on wound healing in rat tongues. Oral Surgery Oral Medicine Oral Pathology Oral Radiology, and Endodontics, 100(3), e58-e63. https://doi.org/10.1016/j.tripleo.2005.04.016

[8] Camacho-Alonso, F., López-Jornet, P., & Bermejo-Fenoll, A. (2005). Effects of scalpel (with and without tissue adhesive) and cryosurgery on wound healing in rat tongues. Oral Surgery Oral Medicine Oral Pathology Oral Radiology, and Endodontics, 100(3), e58-e63. https://doi.org/10.1016/j.tripleo.2005.04.016

[9] Camacho-Alonso, F., Torralba-Ruiz, M. R., García-Carrillo, N., Lacal-Luján, J., Martínez-Díaz, F., & Sánchez-Siles, M. (2019). Effects of topical apllications of porcine acellular urinary bladder matrix and Centella asiatica extract on oral wound healing in a rat model. Clinical Oral Investigations, 23(5), 2083-2095. https://doi.org/10.1007/s00784-018-2620-x

[10] Camacho-Alonso, F., Torralba-Ruiz, M. R., García-Carrillo, N., Lacal-Luján, J., Martínez-Díaz, F., & Sánchez-Siles, M. (2019). Effects of topical apllications of porcine acellular urinary bladder matrix and Centella asiatica extract on oral wound healing in a rat model. Clinical Oral Investigations, 23(5), 2083-2095. https://doi.org/10.1007/s00784-018-2620-x

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Regeneration of lingual musculature in rats using myoblasts over porcine bladder acellular matrix

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Regeneration of lingual musculature in rats using myoblasts over porcine bladder acellular matrix

Authors

Affiliations

Abstract

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References

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MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources