Study presence of COVID-19 (SARS-CoV-2) in the sweat of patients infected with Covid-19

Microb Pathog. 2020 Dec:149:104556. doi: 10.1016/j.micpath.2020.104556. Epub 2020 Oct 5.

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease, which started in Wuhan, Chin, has now become a public health challenge in most countries around the world. Proper preventive measures are necessary to prevent the spread of the virus to help control the pandemic. Because, SARS-CoV-2 is new, its transmission route has not been fully understood. In this study, we aimed to investigate the presence of SARS-CoV-2 in the sweat secretion of COVID-19 patients. Sweat specimens of 25 COVID- 19 patients were collected and tested for SARS-CoV-2 RNA by Real-time Polymerase Chain Reaction (RT-PCR) method. After RNA extraction and cDNA amplification, all samples were examined for the presence of ORF-1ab and N genes related to COVID-19. Results annotated by Realtime PCR machines software based on Dynamic algorithm. The results of this study showed the absence of SARS-CoV-2 in the sweat samples taken from the foreheads of infected people. Therefore, it can be concluded that the sweat of patients with COVID- 19 cannot transmit SARS-CoV-2. However they can be easily contaminated with other body liquids.

Keywords: COVID- 19; Contamination; Detection; Sars-Cov-2; Sweat; Transmission.

MeSH terms

  • Adult
  • COVID-19 / diagnosis
  • COVID-19 / transmission
  • COVID-19 / virology*
  • COVID-19 Testing
  • Female
  • Humans
  • Male
  • Middle Aged
  • RNA, Viral / analysis
  • Real-Time Polymerase Chain Reaction / methods
  • SARS-CoV-2 / genetics
  • SARS-CoV-2 / isolation & purification*
  • Software
  • Sweat / virology*
  • Young Adult

Substances

  • RNA, Viral