The effect of methylcobalamin on the accommodative function of subjects with deteriorated accommodation by induced experimental visual work was studied by measuring small fluctuations of accommodation. The deterioration of small fluctuations of accommodations were difficult to find after visual work in the group receiving methylcobalamin. However, a significant difference of small fluctuations of accommodation was recognized in the non-administered group and in the placebo group (P less than 0.05), and that change was higher in the non-administered group than in the placebo group. Although, the placebo effect of methylcobalamin was undeniable, it was objectively confirmed that methylcobalamin produced a good effect on deteriorated accommodation induced by the visual work.