Pepper vein yellows virus 9: a novel polerovirus isolated from chili pepper in Indonesia

Arch Virol. 2020 Dec;165(12):3017-3021. doi: 10.1007/s00705-020-04838-6. Epub 2020 Oct 10.


In 2017, a leaf sample from a single chili pepper (Capsicum annuum) plant exhibiting yellowing was collected from Aceh province, Indonesia. Total RNA was extracted from this sample, and RNA-Seq analysis was conducted. Putative infecting viruses were detected by mapping the obtained reads to the full-length viral genome sequences available in the GenBank database (7457 sequences) and the de novo-assembled contigs. RNA-Seq analysis detected polerovirus, begomovirus, and amalgavirus sequences, and the polerovirus-like sequences showed strong similarity to those of previously reported pepper vein yellows viruses (PeVYVs). The complete viral genome sequence obtained by RT-PCR had a length of 6023 nt, had the typical genome organization of a polerovirus and showed a high degree of sequence similarity to PeVYV-2 from Israel. Moreover, the predicted amino acid sequence of the P0 protein of the Indonesian isolate was 85.1% to 88.8% identical to those of other PeVYVs. In accordance with the polerovirus species demarcation criteria, this isolate should be assigned to a new polerovirus species, and we propose the name "pepper vein yellows virus 9" (PeVYV-9) for this virus.

MeSH terms

  • Capsicum / virology*
  • Genome, Viral*
  • Indonesia
  • Luteoviridae / classification*
  • Luteoviridae / isolation & purification
  • Phylogeny*
  • Plant Diseases / virology
  • RNA, Viral / genetics
  • Recombination, Genetic
  • Sequence Analysis, DNA


  • RNA, Viral